Matt:LabNotes/2015-1-15: Difference between revisions

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>Mzcai
(Created page with "==CA12k_Nov2014 V4 and V7 Probe Production== *Repeat Matt:LabNotes/2015-1-5 ===Production PCR=== ** 10nM 1st round amplicons *V4...")
 
>Mzcai
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'''Program''' 98C 1min -> (98C 10sec -> 55C 30sec -> 72C 45sec) x 22 -> 72C 2min -> 15C hold
'''Program''' 98C 1min -> (98C 15sec -> 55C 30sec -> 72C 45sec) x 15 -> 72C 2min -> 15C hold
*Stopped after 15 cycles
<!--[[File:010514_CA12kNov14_ProductionPCR_V4V7.JPG | 800px]]
<!--[[File:010514_CA12kNov14_ProductionPCR_V4V7.JPG | 800px]]



Revision as of 20:34, 15 January 2015

CA12k_Nov2014 V4 and V7 Probe Production

Production PCR

Components Volume (1X) Volume (50X)
First round amplicon CA12k_Nov14_V4 (10nM) 0.2 10
2X KAPA SYBG MM 50 2500
AP1V4U (100uM) 0.4 20
AP2V4 (100uM) 0.4 20
H2O 49 2450
Total 100 5000
  • V7 master mix
Components Volume (1X) Volume (50X)
First round amplicon CA12k_Nov14_V7 (10nM) 0.2 10
2X KAPA SYBG MM 50 2500
AP1V7U (100uM) 0.4 20
AP2V7 (100uM) 0.4 20
H2O 49 2450
Total 100 5000

Program 98C 1min -> (98C 15sec -> 55C 30sec -> 72C 45sec) x 15 -> 72C 2min -> 15C hold