Arichard:Samples/sort141009: Difference between revisions

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>Andrew
(Created page with "==2014 October 09 sort for 1-02 AD and 25-01 Control== * 6 tubes, sorted into 1% BSA ** Hi/Lo PI separation only observed in 1-02 CTX * Spun down at 900 RCF for 10 min * Resu...")
 
>Andrew
 
Line 5: Line 5:
* Spun down at 900 RCF for 10 min
* Spun down at 900 RCF for 10 min
* Resuspended each in 100 ul of PBS with 1% BSA and 10% DMSO and counted on BioRad TC20
* Resuspended each in 100 ul of PBS with 1% BSA and 10% DMSO and counted on BioRad TC20
* Made 10 aliquots each of 10 ul and froze down in styrofoam boxes at -80 degC
* Made 10 aliquots each of 10 ul (unless otherwise indicated) and froze down in styrofoam boxes at -80 degC


Tubes
Tubes

Latest revision as of 18:00, 10 February 2015

2014 October 09 sort for 1-02 AD and 25-01 Control[edit]

  • 6 tubes, sorted into 1% BSA
    • Hi/Lo PI separation only observed in 1-02 CTX
  • Spun down at 900 RCF for 10 min
  • Resuspended each in 100 ul of PBS with 1% BSA and 10% DMSO and counted on BioRad TC20
  • Made 10 aliquots each of 10 ul (unless otherwise indicated) and froze down in styrofoam boxes at -80 degC

Tubes

  1. 1-02 CTX Full PI, ~25k sorted, aliquoted @ 1.2e5/ml (in 3 ul)
  2. 1-02 CTX Hi PI, ~25k sorted, aliquoted @ 1.2e5/ml (in 3 ul, 7 tubes)
  3. 1-02 CTX Lo PI, ~25k sorted, aliquoted @ 7.83e4/ml
  4. 1-02 CBL Full PI, ~50k sorted, aliquoted @ 2.74e5/ml
  5. 25-01 CTX Full PI, ~13k sorted, aliquoted @ 7.83e4/m (in 20 ul)
  6. 25-01 CBL Full PI, ~50k sorted, aliquoted @ 2.32e5/ml