Arichard:Notebook/midas150130: Difference between revisions
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==January 1, 2015== | |||
MIDAS on 1-02 (AD) and 25-01 (CTL) | |||
===Prep=== | |||
===Gels=== | |||
Used old 1:50 Nextera on first strip | Used old 1:50 Nextera on first strip | ||
[[File:midas150130Slide1.jpg|400px]] | [[File:midas150130Slide1.jpg|400px]] | ||
Line 20: | Line 28: | ||
[[File:midas150130Slide11.jpg|400px]] | [[File:midas150130Slide11.jpg|400px]] | ||
[[File:midas150130Slide12.jpg|400px]] | [[File:midas150130Slide12.jpg|400px]] | ||
===Sequencing=== | |||
Submitted: | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Array/Sample #''' | |||
| align="center" style="background:#f0f0f0;"|'''Patient''' | |||
| align="center" style="background:#f0f0f0;"|'''NeuN''' | |||
| align="center" style="background:#f0f0f0;"|'''Tissue''' | |||
| align="center" style="background:#f0f0f0;"|'''PI peak''' | |||
| align="center" style="background:#f0f0f0;"|'''Sort date''' | |||
|- | |||
| A2||1-02||+||Cortex||Full 2N||9-Oct-14 | |||
|- | |||
| A3||1-02||+||Cortex||Full 2N||9-Oct-14 | |||
|- | |||
| A4||1-02||+||Cortex||Full 2N||9-Oct-14 | |||
|- | |||
| C19||1-02||+||Cortex||Full 2N||9-Oct-14 | |||
|- | |||
| C20||1-02||+||Cortex||Full 2N||9-Oct-14 | |||
|- | |||
| C21||1-02||+||Cortex||Full 2N||9-Oct-14 | |||
|- | |||
| J33||25-01||+||Cortex||Full 2N||9-Oct-14 | |||
|- | |||
| J35||25-01||+||Cortex||Full 2N||9-Oct-14 | |||
|- | |||
| K41||25-01||+||Cortex||Full 2N||9-Oct-14 | |||
|- | |||
| K44||25-01||+||Cortex||Full 2N||9-Oct-14 | |||
|- | |||
| | |||
|} |
Revision as of 19:42, 2 March 2015
January 1, 2015
MIDAS on 1-02 (AD) and 25-01 (CTL)
Prep
Gels
Used old 1:50 Nextera on first strip File:Midas150130Slide1.jpg File:Midas150130Slide2.jpg
I then tried 1X Tn5059 on strip 2 File:Midas150130Slide3.jpg File:Midas150130Slide4.jpg
Not sure why Tn5059 is not working on these MIDAS amplicons. It may have just been an unlucky batch. I repeated with the remaining samples.
File:Midas150130Slide5.jpg File:Midas150130Slide6.jpg File:Midas150130Slide7.jpg File:Midas150130Slide8.jpg
It looked like these ones worked, so I had Qiming go ahead with bead purification.
File:Midas150130Slide9.jpg File:Midas150130Slide10.jpg File:Midas150130Slide11.jpg File:Midas150130Slide12.jpg
Sequencing
Submitted:
Array/Sample # | Patient | NeuN | Tissue | PI peak | Sort date |
A2 | 1-02 | + | Cortex | Full 2N | 9-Oct-14 |
A3 | 1-02 | + | Cortex | Full 2N | 9-Oct-14 |
A4 | 1-02 | + | Cortex | Full 2N | 9-Oct-14 |
C19 | 1-02 | + | Cortex | Full 2N | 9-Oct-14 |
C20 | 1-02 | + | Cortex | Full 2N | 9-Oct-14 |
C21 | 1-02 | + | Cortex | Full 2N | 9-Oct-14 |
J33 | 25-01 | + | Cortex | Full 2N | 9-Oct-14 |
J35 | 25-01 | + | Cortex | Full 2N | 9-Oct-14 |
K41 | 25-01 | + | Cortex | Full 2N | 9-Oct-14 |
K44 | 25-01 | + | Cortex | Full 2N | 9-Oct-14 |