Matt:LabNotes/2015-5-5: Difference between revisions

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>Mzcai
(Created page with "==CA12k_Nov2014_V4 Probe Production== *Production of Padlock Probes (V4) Matt:LabNotes/2014-11-19#Assembly_of_oligo_pool_for_ordering ===Production PCR=== *[[Matt:LabNote...")
 
>Mzcai
mNo edit summary
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| align="center" style="background:#f0f0f0;"|'''Volume (100X)'''
| align="center" style="background:#f0f0f0;"|'''Volume (100X)'''
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| First round amplicon CA12k_Nov14_V7 (10nM)||0.2||20
| First round amplicon CA12k_Nov14_V4 (10nM)||0.2||20
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| 2X KAPA SYBG MM||50||5000
| 2X KAPA SYBG MM||50||5000

Revision as of 20:14, 5 May 2015

CA12k_Nov2014_V4 Probe Production

Production PCR

Components Volume (1X) Volume (100X)
First round amplicon CA12k_Nov14_V4 (10nM) 0.2 20
2X KAPA SYBG MM 50 5000
AP1V4U (100uM) 0.4 40
AP2V4 (100uM) 0.4 40
H2O 49 4900
Total 100 10000

Program 98C 1min -> (98C 10sec -> 55C 30sec -> 72C 45sec) x 15 -> 72C 2min -> 15C hold