Sam:LabNotes/Microbione/2009-3-3/exp1: Difference between revisions

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>Sam Chiang
>Sam Chiang
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===Slide washing===
===Slide washing (Tween 20/PBS washing) ===
 
#Add 20 uL of Tween 20 to 180 uL of H20 (10% dilution).
#Add 19.2 mL of PBS to the solution for a final dilution of .1% Tween 20. 
#Shake until a soapy consistency is observed
#Gently spray 5 ml of the solution over the microarrays
#Wipe carefully with a kimwipe and allow to dry


===Beads loading===
===Beads loading===

Revision as of 17:45, 5 March 2009

Slide washing (Tween 20/PBS washing)

  1. Add 20 uL of Tween 20 to 180 uL of H20 (10% dilution).
  2. Add 19.2 mL of PBS to the solution for a final dilution of .1% Tween 20.
  3. Shake until a soapy consistency is observed
  4. Gently spray 5 ml of the solution over the microarrays
  5. Wipe carefully with a kimwipe and allow to dry

Beads loading

Microscope image

  • Beads in 1/100 dilution, objective 20X (left); Beads in 1/100 dilution, objective 10X (right)
  File:Bead-3-4-09 1-100 2.bmp    File:Bead-3-4-09 1-100.bmp 


  • Beads in 1/200 dilution, objective 20X (left); Beads in 1/200 dilution, objective 20X (right)
  File:Bead-3-4-09 1-200 2.bmp    File:Bead-3-4-09 1-200.bmp