Sam:LabNotes/Microbione/2009-3-3/exp1: Difference between revisions
Jump to navigation
Jump to search
>Sam Chiang |
>Sam Chiang |
||
Line 13: | Line 13: | ||
#Firmly press down until the squares are sealed | #Firmly press down until the squares are sealed | ||
#Remove the protective covering from the other side and press down, ensuring a tight seal | #Remove the protective covering from the other side and press down, ensuring a tight seal | ||
===Microscope image=== | ===Microscope image=== |
Revision as of 17:45, 5 March 2009
Slide washing (Tween 20/PBS washing)
- Add 20 uL of Tween 20 to 180 uL of H20 (10% dilution).
- Add 19.2 mL of PBS to the solution for a final dilution of .1% Tween 20.
- Shake until a soapy consistency is observed
- Gently spray 5 ml of the solution over the microarrays
- Wipe carefully with a kimwipe and allow to dry
Beads loading
- Remove the protective covering from one side of the gasket and place in the desired position on the microarray
- Firmly press down until the squares are sealed
- Remove the protective covering from the other side and press down, ensuring a tight seal
Microscope image
- Beads in 1/100 dilution, objective 20X (left); Beads in 1/100 dilution, objective 10X (right)
File:Bead-3-4-09 1-100 2.bmp File:Bead-3-4-09 1-100.bmp
- Beads in 1/200 dilution, objective 20X (left); Beads in 1/200 dilution, objective 20X (right)
File:Bead-3-4-09 1-200 2.bmp File:Bead-3-4-09 1-200.bmp