Matt:LabNotes/2015-6-5: Difference between revisions
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===RNA FISH=== | ===RNA FISH=== | ||
[[Daniel:Notebook/RNAFISH/2015-3-4 | Dan's best practice FISH protocol]] | [[Daniel:Notebook/RNAFISH/2015-3-4 | Dan's best practice FISH protocol]]<br> | ||
[[Daniel:Protocols/TissueFISH#Linnarsson_Lab_Protocol | Linnarsson complete FISH protocol]] | [[Daniel:Protocols/TissueFISH#Linnarsson_Lab_Protocol | Linnarsson complete FISH protocol]] | ||
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Revision as of 02:17, 5 June 2015
RNA FISH: Probe Resuspension and Dye Coupling
Probe Resuspension
- The 5' amino modified (separated by C6) probes ordered by Dan:
- GAD1
- Already resuspended
- FOXP2
- Already resuspended
- CUX2
- Already resuspended
- SLC6A1
- ADARB2
- SATB2
- KIT
- GAD1
- Pick 2-3 genes and resuspend if needed
Dye Coupling
Dan's best practice dye coupling protocol Generic dye coupling protocol