Sam:LabNotes/Microbiome/2009-3-9: Difference between revisions
Jump to navigation
Jump to search
>Sam Chiang (New page: ='''E. coli labeling using SYBR Green I and YOYO-1'''= ==Objective== *To confirm the bacteria staining with revised procedures **Using FITC filter, 20X objective, No cover slip *The sta...) |
>Sam Chiang |
||
Line 24: | Line 24: | ||
*Mix the E. coli with either 10 uL 2X SYBR Green I or 2 uL of YOYo-1 dye, gently pipeting for mixing | *Mix the E. coli with either 10 uL 2X SYBR Green I or 2 uL of YOYo-1 dye, gently pipeting for mixing | ||
*Leave the slide in dark box (slide storage box) | *Leave the slide in dark box (slide storage box)for incubation 15 min in room temp | ||
==Results== | |||
[[Image:SNAP-151939-0309-sybr-20x-B&W.jpg|SNAP-151939-0309-sybr-20x-B&W]] | |||
[[Image:SNAP-153406-0004-sybr-20x-fitc.jpg|SNAP-153406-0004-sybr-20x-fitc]] | |||
[[Image:SNAP-153514-0005-sybr-20x-fitc.jpg|SNAP-153514-0005-sybr-20x-fitc]] |
Revision as of 23:52, 9 March 2009
E. coli labeling using SYBR Green I and YOYO-1
Objective
- To confirm the bacteria staining with revised procedures
- Using FITC filter, 20X objective, No cover slip
- The stained bacteria will be used for microwell application
Material and Samples
- Samples: Diluted E. coli, 10^6 CFU/uL (stored in PBS, 4C)
- YOYO®-1 iodide (491/509) - 1 mM solution in DMSO
- Diluted SYBR Green I (diluted in H2O, stored in 4C)
Procedures
- Drop 1 uL of E. coli on the glass slide
- Mix the E. coli with either 10 uL 2X SYBR Green I or 2 uL of YOYo-1 dye, gently pipeting for mixing
- Leave the slide in dark box (slide storage box)for incubation 15 min in room temp
Results
SNAP-151939-0309-sybr-20x-B&W SNAP-153406-0004-sybr-20x-fitc SNAP-153514-0005-sybr-20x-fitc