Sam:LabNotes/Microbiome/2009-3-9 exp2: Difference between revisions
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*Based on the previous multiple PCR result, increase the primer concentration for those weak bands | *Based on the previous multiple PCR result, increase the primer concentration for those weak bands | ||
*Test the effect of Q-solution come with Qiagen multiple PCR kit | *Test the effect of Q-solution come with Qiagen multiple PCR kit | ||
==Material and Samples== | ==Material and Samples== | ||
*Prepare the new primer gourp mix for primer set-1(1X = 10 uL, 2X = 20 uL) | *Prepare the new primer gourp mix for primer set-1(1X = 10 uL, 2X = 20 uL) | ||
Set-1 Blue: including Y-1(2X), 21-1(2X), the rest primers (1X) | **Set-1 Blue: including Y-1(2X), 21-1(2X), the rest primers (1X) | ||
Set-1 Yellow: 11-1(2X), 10-1(2X), 16-1(2X) | **Set-1 Yellow: 11-1(2X), 10-1(2X), 16-1(2X) | ||
Set-1 Red: Use the old one | **Set-1 Red: Use the old one | ||
Set-1 Green: 18-1(2X), 3-1(2X) | **Set-1 Green: 18-1(2X), 3-1(2X) | ||
Set-1 Orange: 20-1(2X), 7-1(2X) | **Set-1 Orange: 20-1(2X), 7-1(2X) | ||
==Procedures== |
Revision as of 04:13, 10 March 2009
Multiple PCR against human whole genomoe
Objective
- Based on the previous multiple PCR result, increase the primer concentration for those weak bands
- Test the effect of Q-solution come with Qiagen multiple PCR kit
Material and Samples
- Prepare the new primer gourp mix for primer set-1(1X = 10 uL, 2X = 20 uL)
- Set-1 Blue: including Y-1(2X), 21-1(2X), the rest primers (1X)
- Set-1 Yellow: 11-1(2X), 10-1(2X), 16-1(2X)
- Set-1 Red: Use the old one
- Set-1 Green: 18-1(2X), 3-1(2X)
- Set-1 Orange: 20-1(2X), 7-1(2X)