Andrew:Notebook/AR150817 C1: Difference between revisions
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===MAPD profiling=== | ===MAPD profiling=== | ||
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Latest revision as of 23:05, 14 September 2015
Prep[edit]
Protocol[edit]
File:AR150817 reagents-page-001.jpg
Results[edit]
C1 capture[edit]
Chamber | Number | LiveDead | Note | Well |
Ch03 | 1 | live | extravasating | A01 |
Ch09 | 1 | dead | na | B01 |
Ch15 | multi | dead | na | C01 |
Ch21 | 0 | na | na | D01 |
Ch25 | mutli | both | na | E01 |
Ch31 | 0 | na | na | F01 |
Ch37 | 1 | dead | na | G01 |
Ch43 | 0 | na | na | H01 |
Ch02 | 1 | dead | na | A02 |
Ch08 | 1 | dead | extravasating | B02 |
Ch14 | 1 | both | weak | C02 |
Ch20 | 0 | na | na | D02 |
Ch26 | mutli | both | na | E02 |
Ch32 | muti | both | na | F02 |
Ch38 | 0 | na | na | G02 |
Ch44 | 1 | live | extravasating | H02 |
Ch01 | 1 | dead | extravasating | A03 |
Ch07 | 1 | dead | na | B03 |
Ch13 | 1 | both | extravasating | C03 |
Ch19 | 0 | na | na | D03 |
Ch27 | 1 | live | extravasating | E03 |
Ch33 | 1 | dead | debris | F03 |
Ch39 | multi | both | na | G03 |
Ch45 | 1 | dead | na | H03 |
Ch49 | 1 | live | weak | A04 |
Ch55 | 0 | na | na | B04 |
Ch61 | 1 | dead | na | C04 |
Ch67 | 0 | na | na | D04 |
Ch75 | 0 | na | na | E04 |
Ch81 | 0 | na | na | F04 |
Ch87 | 0 | na | na | G04 |
Ch93 | 0 | na | na | H04 |
Ch50 | multi | live | na | A05 |
Ch56 | 0 | na | na | B05 |
Ch62 | mutli | dead | debris | C05 |
Ch68 | 1 | live | extravasating | D05 |
Ch74 | 0 | na | na | E05 |
Ch80 | 1 | dead | na | F05 |
Ch86 | 0 | na | na | G05 |
Ch92 | multi | live | na | H05 |
Ch51 | 0 | na | na | A06 |
Ch57 | 1 | dead | extravasating | B06 |
Ch63 | 1 | live | extravasating | C06 |
Ch69 | multi | dead | na | D06 |
Ch73 | 1 | both | na | E06 |
Ch79 | 0 | na | na | F06 |
Ch85 | 1 | dead | na | G06 |
Ch91 | 0 | na | na | H06 |
Ch06 | 1 | dead | weak | A07 |
Ch12 | 1 | live | extravasating | B07 |
Ch18 | 0 | dead | weak_debris | C07 |
Ch24 | 1 | live | extravasating | D07 |
Ch28 | 0 | na | na | E07 |
Ch34 | 0 | na | na | F07 |
Ch40 | 1 | live | na | G07 |
Ch46 | 1 | live | extravasating | H07 |
Ch05 | 0 | dead | debris | A08 |
Ch11 | 1 | live | extravasating | B08 |
Ch17 | 0 | na | na | C08 |
Ch23 | 0 | na | na | D08 |
Ch29 | 1 | live | weak | E08 |
Ch35 | 0 | na | na | F08 |
Ch41 | 1 | dead | debris | G08 |
Ch47 | 1 | live | weak | H08 |
Ch04 | multi | dead | na | A09 |
Ch10 | mutli | both | na | B09 |
Ch16 | multi | live | na | C09 |
Ch22 | mutli | dead | weak_debris | D09 |
Ch30 | 1 | live | extravasating | E09 |
Ch36 | 1 | dead | na | F09 |
Ch42 | 1 | live | weak | G09 |
Ch48 | multi | both | na | H09 |
Ch52 | 1 | dead | na | A10 |
Ch58 | 0 | na | na | B10 |
Ch64 | 0 | na | na | C10 |
Ch70 | 0 | na | na | D10 |
Ch78 | 1 | dead | na | E10 |
Ch84 | 0 | na | na | F10 |
Ch90 | 0 | na | na | G10 |
Ch96 | multi | live | na | H10 |
Ch53 | 0 | na | na | A11 |
Ch59 | mutli | dead | weak | B11 |
Ch65 | 1 | live | weak | C11 |
Ch71 | multi | both | na | D11 |
Ch77 | 0 | na | na | E11 |
Ch83 | 0 | na | na | F11 |
Ch89 | 0 | na | na | G11 |
Ch95 | 0 | na | na | H11 |
Ch54 | 0 | na | na | A12 |
Ch60 | 1 | live | extravasating | B12 |
Ch66 | 0 | na | na | C12 |
Ch72 | 1 | live | extravasating | D12 |
Ch76 | 0 | na | na | E12 |
Ch82 | 0 | na | na | F12 |
Ch88 | 1 | dead | debris_weak | G12 |
Ch94 | 1 | both | na | H12 |
hLINE qPCR[edit]
Size selection[edit]
Sequencing[edit]
Chamber | Well | Number | LiveDead | Note | Cq | pg/uL | N7xx | S5xx | i7 | i5 | SampleID |
Ch24 | D07 | 1 | live | extravasating | 17.22395 | 261.0341321 | N712 | S517 | GTAGAGGA | GCGTAAGA | AR-150817-Ch24-D07-N712-S517 |
Ch27 | E03 | 1 | live | extravasating | 17.19854949 | 265.4277765 | N712 | S502 | GTAGAGGA | CTCTCTAT | AR-150817-Ch27-E03-N712-S502 |
Ch40 | G07 | 1 | live | na | 16.86216898 | 331.0901587 | N712 | S503 | GTAGAGGA | TATCCTCT | AR-150817-Ch40-G07-N712-S503 |
Ch44 | H02 | 1 | live | extravasating | 16.43120196 | 439.4811917 | N712 | S504 | GTAGAGGA | AGAGTAGA | AR-150817-Ch44-H02-N712-S504 |
Ch63 | C06 | 1 | live | extravasating | 16.31240839 | 475.1632934 | N712 | S505 | GTAGAGGA | GTAAGGAG | AR-150817-Ch63-C06-N712-S505 |
Ch30 | E09 | 1 | live | extravasating | 16.26635684 | 489.7625484 | N712 | S506 | GTAGAGGA | ACTGCATA | AR-150817-Ch30-E09-N712-S506 |
Ch78dea | E10 | 1 | dead | na | 18.34499955 | 124.9568512 | N712 | S507 | GTAGAGGA | AAGGAGTA | AR-150817-Ch78dea-E10-N712-S507 |
Ch21ntc | D01 | 0 | na | na | 25.93584616 | 0.851926856 | N712 | S508 | GTAGAGGA | CTAAGCCT | AR-150817-Ch21ntc-D01-N712-S508 |
CNV profiling[edit]
File:AR-150817-Ch21ntc-D01-N712-S508.all.png File:AR-150817-Ch24-D07-N712-S517.all.png File:AR-150817-Ch27-E03-N712-S502.all.png File:AR-150817-Ch30-E09-N712-S506.all.png File:AR-150817-Ch40-G07-N712-S503.all.png File:AR-150817-Ch44-H02-N712-S504.all.png File:AR-150817-Ch63-C06-N712-S505.all.png File:AR-150817-Ch78dea-E10-N712-S507.all.png
MAPD profiling[edit]
File:AR-150817-Ch21ntc-D01-N712-S508.jpg mapd File:AR-150817-Ch24lve-D07-N712-S517.jpg mapd File:AR-150817-Ch27lve-E03-N712-S502.jpg mapd File:AR-150817-Ch30lve-E09-N712-S506.jpg mapd File:AR-150817-Ch40lve-G07-N712-S503.jpg mapd File:AR-150817-Ch44lve-H02-N712-S504.jpg mapd File:AR-150817-Ch63lve-C06-N712-S505.jpg mapd File:AR-150817-Ch78dea-E10-N712-S507.jpg mapd