Alice:LabNotes/2007-11-11: Difference between revisions
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14 samples from experiment 1 was sent out for sequencing on Nov 5th, 2007 (sample #3 and 12 were excluded because the reactions were not successful). Since we are particularly interested in sample #14 (rs1264899), two duplicates of this sample were also included (see lab note on Nov-2-07). The sequencing result shows that sample #1,2 (rs3753565), 3 (rs2295680), 12,14,16 (rs 1264899) had multiple peaks on every base pair, which indicates these had bad results. However, SNP locations were successfully found for sample #13 and 15. | 14 samples from experiment 1 was sent out for sequencing on Nov 5th, 2007 (sample #3 and 12 were excluded because the reactions were not successful). Since we are particularly interested in sample #14 (rs1264899), two duplicates of this sample were also included (see lab note on Nov-2-07). The sequencing result shows that sample #1,2 (rs3753565), 3 (rs2295680), 12,14,16 (rs 1264899) had multiple peaks on every base pair, which indicates these had bad results. However, SNP locations were successfully found for sample #13 and 15. | ||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Sample''' | |||
| align="center" style="background:#f0f0f0;"|'''Sequencing result''' | |||
|- | |||
| 1 ||no || | |||
|- | |||
| 2 ||no || | |||
|- | |||
| 3 ||no || | |||
|- | |||
| 4 ||yes || | |||
|- | |||
| 5 ||yes || | |||
|- | |||
| 6 ||yes || | |||
|- | |||
| 7 ||yes || | |||
|- | |||
| 8 ||yes || | |||
|- | |||
| 9 ||yes || | |||
|- | |||
| 10 ||yes || | |||
|- | |||
| 11 ||yes || | |||
|- | |||
| 12 ||no || | |||
|- | |||
| 13 ||yes || | |||
|- | |||
| 14 ||no || | |||
|- | |||
| 15 ||yes || | |||
|- | |||
| 16 ||no || | |||
|- | |||
| | |||
|} | |||
<b>Sequencing Results:</b><br> | <b>Sequencing Results:</b><br> | ||
The SNP locations for sample #13 and #15 were determined using Genetool and Sequence Scanner, and the result is as follows: <br> | The SNP locations for sample #13 and #15 were determined using Genetool and Sequence Scanner, and the result is as follows: <br> |
Latest revision as of 05:14, 3 December 2007
14 samples from experiment 1 was sent out for sequencing on Nov 5th, 2007 (sample #3 and 12 were excluded because the reactions were not successful). Since we are particularly interested in sample #14 (rs1264899), two duplicates of this sample were also included (see lab note on Nov-2-07). The sequencing result shows that sample #1,2 (rs3753565), 3 (rs2295680), 12,14,16 (rs 1264899) had multiple peaks on every base pair, which indicates these had bad results. However, SNP locations were successfully found for sample #13 and 15.
Sample | Sequencing result | |
1 | no | |
2 | no | |
3 | no | |
4 | yes | |
5 | yes | |
6 | yes | |
7 | yes | |
8 | yes | |
9 | yes | |
10 | yes | |
11 | yes | |
12 | no | |
13 | yes | |
14 | no | |
15 | yes | |
16 | no | |
Sequencing Results:
The SNP locations for sample #13 and #15 were determined using Genetool and Sequence Scanner, and the result is as follows:
SNP: rs1264899
Primer: rs1264899_cF/rs1264899_cR
Primer location: Hues6Ver2c F1/F4
Sample #13:
Template: PGP1 gDNA
File:SNP.png
Sample #15:
Template: GC1F cDNA
File:SNP2.png