Alice:LabNotes/2007-11-11: Difference between revisions

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14 samples from experiment 1 was sent out for sequencing on Nov 5th, 2007 (sample #3 and 12 were excluded because the reactions were not successful). Since we are particularly interested in sample #14 (rs1264899), two duplicates of this sample were also included (see lab note on Nov-2-07). The sequencing result shows that sample #1,2 (rs3753565), 3 (rs2295680), 12,14,16 (rs 1264899) had multiple peaks on every base pair, which indicates these had bad results. However, SNP locations were successfully found for sample #13 and 15.  
14 samples from experiment 1 was sent out for sequencing on Nov 5th, 2007 (sample #3 and 12 were excluded because the reactions were not successful). Since we are particularly interested in sample #14 (rs1264899), two duplicates of this sample were also included (see lab note on Nov-2-07). The sequencing result shows that sample #1,2 (rs3753565), 3 (rs2295680), 12,14,16 (rs 1264899) had multiple peaks on every base pair, which indicates these had bad results. However, SNP locations were successfully found for sample #13 and 15.  
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''Sample'''
| align="center" style="background:#f0f0f0;"|'''Sequencing result'''
|-
| 1 ||no ||
|-
| 2 ||no ||
|-
| 3 ||no ||
|-
| 4 ||yes ||
|-
| 5 ||yes ||
|-
| 6 ||yes ||
|-
| 7 ||yes ||
|-
| 8 ||yes ||
|-
| 9 ||yes ||
|-
| 10 ||yes ||
|-
| 11 ||yes ||
|-
| 12 ||no ||
|-
| 13 ||yes ||
|-
| 14 ||no ||
|-
| 15 ||yes ||
|-
| 16 ||no ||
|-
|
|}
<b>Sequencing Results:</b><br>
<b>Sequencing Results:</b><br>
The SNP locations for sample #13 and #15 were determined using Genetool and Sequence Scanner, and the result is as follows: <br>
The SNP locations for sample #13 and #15 were determined using Genetool and Sequence Scanner, and the result is as follows: <br>

Latest revision as of 05:14, 3 December 2007

14 samples from experiment 1 was sent out for sequencing on Nov 5th, 2007 (sample #3 and 12 were excluded because the reactions were not successful). Since we are particularly interested in sample #14 (rs1264899), two duplicates of this sample were also included (see lab note on Nov-2-07). The sequencing result shows that sample #1,2 (rs3753565), 3 (rs2295680), 12,14,16 (rs 1264899) had multiple peaks on every base pair, which indicates these had bad results. However, SNP locations were successfully found for sample #13 and 15.

Sample Sequencing result
1 no
2 no
3 no
4 yes
5 yes
6 yes
7 yes
8 yes
9 yes
10 yes
11 yes
12 no
13 yes
14 no
15 yes
16 no

Sequencing Results:
The SNP locations for sample #13 and #15 were determined using Genetool and Sequence Scanner, and the result is as follows:
SNP: rs1264899
Primer: rs1264899_cF/rs1264899_cR
Primer location: Hues6Ver2c F1/F4
Sample #13:
Template: PGP1 gDNA
File:SNP.png
Sample #15:
Template: GC1F cDNA
File:SNP2.png