Daniel:Notebook/ComboLock: Difference between revisions
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>Djacobse (Created page with "=Combo-Lock= This page describes experiments done for the Combo-lock project. The overview of the project is to encapsulate cells (or nuclei) in droplets using a Drop Seq typ...") |
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This page describes experiments done for the Combo-lock project. The overview of the project is to encapsulate cells (or nuclei) in droplets using a Drop Seq type device. The device will include bis/acrylamide gel formation, and formaldehyde to bond the nucleotides/protein to e gel matrix. following that, attachment of barcoded RNA/antibodyprobes will occur. The antibodies will contain nucleotide barcodes. The nucleotide barcodes will be read by universl pdlock probes, which will then be ligated/cicularized. following ligation, RCA will be performed, and the RCA product isolated for sequencing. | This page describes experiments done for the Combo-lock project. The overview of the project is to encapsulate cells (or nuclei) in droplets using a Drop Seq type device. The device will include bis/acrylamide gel formation, and formaldehyde to bond the nucleotides/protein to e gel matrix. following that, attachment of barcoded RNA/antibodyprobes will occur. The antibodies will contain nucleotide barcodes. The nucleotide barcodes will be read by universl pdlock probes, which will then be ligated/cicularized. following ligation, RCA will be performed, and the RCA product isolated for sequencing. |
Revision as of 18:58, 9 March 2016
Combo-Lock
This page describes experiments done for the Combo-lock project. The overview of the project is to encapsulate cells (or nuclei) in droplets using a Drop Seq type device. The device will include bis/acrylamide gel formation, and formaldehyde to bond the nucleotides/protein to e gel matrix. following that, attachment of barcoded RNA/antibodyprobes will occur. The antibodies will contain nucleotide barcodes. The nucleotide barcodes will be read by universl pdlock probes, which will then be ligated/cicularized. following ligation, RCA will be performed, and the RCA product isolated for sequencing.