Daniel:Notebook/ComboLock: Difference between revisions
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[[Daniel Jacobsen|Back to Main]] | [[Daniel Jacobsen|Back to Main]] | ||
This page describes experiments done for the Combo-lock project. The overview of the project is to encapsulate cells (or nuclei) in droplets using a Drop Seq type device. The device will include bis/acrylamide gel formation, and formaldehyde to bond the nucleotides/protein to e gel matrix. following that, attachment of barcoded RNA/ | This page describes experiments done for the Combo-lock project. The overview of the project is to encapsulate cells (or nuclei) in droplets using a Drop Seq type device. The device will include bis/acrylamide gel formation, and formaldehyde to bond the nucleotides/protein to e gel matrix. following that, attachment of barcoded RNA/antibody probes will occur. The antibodies will contain nucleotide barcodes. The nucleotide barcodes will be read by universal padlock probes, which will then be ligated/cicularized. following ligation, RCA will be performed, and the RCA product isolated for sequencing. | ||
==Assorted Lab Notes== | ==Assorted Lab Notes== |
Revision as of 17:17, 21 April 2016
Combo-Lock
This page describes experiments done for the Combo-lock project. The overview of the project is to encapsulate cells (or nuclei) in droplets using a Drop Seq type device. The device will include bis/acrylamide gel formation, and formaldehyde to bond the nucleotides/protein to e gel matrix. following that, attachment of barcoded RNA/antibody probes will occur. The antibodies will contain nucleotide barcodes. The nucleotide barcodes will be read by universal padlock probes, which will then be ligated/cicularized. following ligation, RCA will be performed, and the RCA product isolated for sequencing.
Assorted Lab Notes
Calendar
<calendar> name=Daniel:Notebook/ComboLock format=%name/%year-%month-%day date=2016/02/01 view=oneyear </calendar>