Daniel:Notebook/ComboLock/Devices: Difference between revisions

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Some specs for the Drop Seq device from the [http://www.cell.com/abstract/S0092-8674%2815%2900549-8 Macosko et al 2013 paper].  Below is an image of a single device.  A chip contains several dozen of these devices.
Some specs for the Drop Seq device from the [http://www.cell.com/abstract/S0092-8674%2815%2900549-8 Macosko et al 2013 paper].  Below is an image of a single device.  A chip contains several dozen of these devices.
[[Image:Drop_Seq_Device.png|thumb|200 px]]
As can be seen, 4 lines are used, 3 inlets and an outlet.  Two of the inlets are aqueous, the third is oil for droplet formation.
[[Image:DropSeqDevice_Junctions.png|thumb|200px]]
The second thumbnail image shows the drop seq device junctions more closely.  The first junction combines the two aqueous solutions; in the original paper this combines a cell solution with beads in a lysis buffer solution.  See Combo-Lock protocols for details on which solutions I use.  The second junction combines the mixed aqueous phases with the oil phase.  This is where droplet formation occurs.  Note that post-mixing there are grooves in the device.  This facilitates mixing.


<gallery heights=400px widths=400px perrow=2 caption="Cross-Sectional Images of the Drop Seq Device">
<gallery heights=400px widths=400px perrow=2 caption="Cross-Sectional Images of the Drop Seq Device">
File:Drop_Seq_Device.png|Device inlets and outlets. As can be seen, 4 lines are used, 3 inlets and an outlet.  Two of the inlets are aqueous, the third is oil for droplet formation.
File:Drop_Seq_Device.png|'''Device inlets and outlets'''. As can be seen, 4 lines are used, 3 inlets and an outlet.  Two of the inlets are aqueous, the third is oil for droplet formation.
File:DropSeqDevice_Junctions.png|Drop seq device junctions.  The first junction combines the two aqueous solutions; in the original paper this combines a cell solution with beads in a lysis buffer solution.  See Combo-Lock protocols for further details.  The second junction combines the mixed aqueous phases with the oil phase, forming droplets.  Note the post-mixing grooves to facilitate mixing.
File:DropSeqDevice_Junctions.png|'''Drop seq device junctions'''.  The first junction combines the two aqueous solutions; in the original paper this combines a cell solution with beads in a lysis buffer solution.  See Combo-Lock protocols for further details.  The second junction combines the mixed aqueous phases with the oil phase, forming droplets.  Note the post-mixing grooves to facilitate mixing.
</gallery>
</gallery>

Latest revision as of 17:58, 12 March 2016

Microfluidic Devices for Combo-Lock[edit]

Back to Notebook

This page details the microfluidic devices used for the Combo-lock project. I have tried to include specifications whenever possible.

Drop Seq Device[edit]

Some specs for the Drop Seq device from the Macosko et al 2013 paper. Below is an image of a single device. A chip contains several dozen of these devices.