Daniel:Notebook/ComboLock/2016-3-15: Difference between revisions
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>Djacobse (Created page with "=10% Acrylamide VA-044= Back to Calendar From yesterday's results we decided to increase the acrylamide...") |
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| align="center" align="center" valign="bottom" | 40% | | align="center" align="center" valign="bottom" | 40% | ||
| align="center" align="center" valign="bottom" | 9% | | align="center" align="center" valign="bottom" | 9% | ||
| align="center" valign="bottom" | | | align="center" valign="bottom" | 6.75 mL | ||
|- style="background-color:#D9D9D9;font-size:12pt" | |- style="background-color:#D9D9D9;font-size:12pt" | ||
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| align="center" valign="bottom" | 40% (38:2) | | align="center" valign="bottom" | 40% (38:2) | ||
| align="center" valign="bottom" | 0.95:0.05 (%) | | align="center" valign="bottom" | 0.95:0.05 (%) | ||
| align="center" valign="bottom" | | | align="center" valign="bottom" | 0.75 mL | ||
|- style="font-size:12pt" | |- style="font-size:12pt" | ||
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| align="center" valign="bottom" | ----- | | align="center" valign="bottom" | ----- | ||
| align="center" valign="bottom" | 0.25% (m/v) | | align="center" valign="bottom" | 0.25% (m/v) | ||
| align="center" valign="bottom" | 0. | | align="center" valign="bottom" | 0.075 g | ||
|- style="background-color:#D9D9D9;font-size:12pt" | |- style="background-color:#D9D9D9;font-size:12pt" | ||
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| align="center" valign="bottom" | 10X | | align="center" valign="bottom" | 10X | ||
| align="center" valign="bottom" | 1X | | align="center" valign="bottom" | 1X | ||
| align="center" valign="bottom" | | | align="center" valign="bottom" | 3 mL | ||
|- style="background-color:#D9D9D9;font-size:12pt" | |- style="background-color:#D9D9D9;font-size:12pt" | ||
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| align="center" valign="bottom" | ----- | | align="center" valign="bottom" | ----- | ||
| align="center" valign="bottom" | ----- | | align="center" valign="bottom" | ----- | ||
| align="center" valign="bottom" | | | align="center" valign="bottom" | 9.5 mL | ||
|- style="font-size:12pt" | |- style="font-size:12pt" | ||
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| align="center" align="center" valign="bottom" | | | align="center" align="center" valign="bottom" | | ||
| align="center" align="center" valign="bottom" | | | align="center" align="center" valign="bottom" | | ||
|style="font-weight:bold" align="center" valign="bottom" | | |style="font-weight:bold" align="center" valign="bottom" | 30 mL | ||
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Revision as of 21:32, 15 March 2016
10% Acrylamide VA-044
From yesterday's results we decided to increase the acrylamide concentration in the gel solution in order to make up for a lack of formaldehyde/tissue.
VA-044/10% Acrylamide test
For test 2 we used the same setup as test 1, but with the following recipe for the VA-044 gel mix:
Reagent | Stock Concentration | Final Concentration | Amount added |
Acrylamide | 40% | 9% | 6.75 mL |
Acrylamide:Bis (19:1) | 40% (38:2) | 0.95:0.05 (%) | 0.75 mL |
VA-044 | ----- | 0.25% (m/v) | 0.075 g |
PBS | 10X | 1X | 3 mL |
ddH2O | ----- | ----- | 9.5 mL |
Total | 30 mL |
Run Parameters
- Line 1: Oil (on ice); X psi; ~2 mL
- Line 2: Aqueous (VA-044P; on ice); X psi; ~1 mL