Daniel:Protocols/AbHybridization: Difference between revisions

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(Created page with "=Antibody Hybridization= Back to Notebook This page details the protocol from [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4713867/ Darmanis et al paper]...")
 
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<li>Mix 1 uL cell lysate with 3 uL antibody mix</li>
<li>Mix 1 uL cell lysate with 3 uL antibody mix</li>
<li>Antibody Mix Contains</li>
<li>Antibody Mix Contains</li>
<ol start="A">
<ol type="A">
<li>0.3 uL Probe A</li>
<li>0.3 uL Probe A</li>
<li>0.3 uL Probe B</li>
<li>0.3 uL Probe B</li>

Revision as of 21:21, 6 May 2016

Antibody Hybridization

Back to Notebook

This page details the protocol from Darmanis et al paper. The protocol is for hybridizing antibodies linked with oligonucleotides to their protein targets. The protocol will be used in validation experiments for Combo-Lock.

Darmanis Protocol

  1. Mix 1 uL cell lysate with 3 uL antibody mix
  2. Antibody Mix Contains
    1. 0.3 uL Probe A
    2. 0.3 uL Probe B
    3. 0.2 uL Incubation Stabilizer (Olink Bioscience)
    4. 2.1 uL Incubation Solution (Olink Biosicence)
  3. Centrifuge samples and incubate overnight at 4C