CRISPR Library Design Pipeline: Difference between revisions
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The process for designing the [[05/05/2015 Library Order]] and [[02/17/2016 Library Order]] included: | The process for designing the [[05/05/2015 Library Order]] and [[02/17/2016 Library Order]] included: | ||
#Create a manually curated gene list for each library. | #Create a manually curated gene list for each library. | ||
#Pick 3-4 gRNAs per gene with the highest score from this [[File: gRNA_spacer_file.txt]] | #Pick 3-4 gRNAs per gene with the highest score from this [[File:gRNA_spacer_file.txt]] | ||
#Add scaffold sequences and desired barcodes. The barcode list can be found in [[File: | #Add scaffold sequences and desired barcodes. The barcode list can be found in [[File:gRNA_bc25mers.txt]]. The scaffold sequences can be found on this page: [[gRNA Scaffold Sequences]]. |
Revision as of 06:33, 21 May 2016
The process for designing the 05/05/2015 Library Order and 02/17/2016 Library Order included:
- Create a manually curated gene list for each library.
- Pick 3-4 gRNAs per gene with the highest score from this File:GRNA spacer file.txt
- Add scaffold sequences and desired barcodes. The barcode list can be found in File:GRNA bc25mers.txt. The scaffold sequences can be found on this page: gRNA Scaffold Sequences.