Alice:LabNotes/2007-11-26: Difference between revisions
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Each well contain total of 5 ul of mixture of reagents.<br> | |||
<b>Summary of volumes of Reagents used:</b> | <b>Summary of volumes of Reagents used:</b> | ||
{| {{table}} | {| {{table}} | ||
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<b>Results:</b><br> | <b>Results:</b><br> | ||
[[Image:ZhangLab_2 2007-11-26 13hr 17min-2-.png]] <br> | [[Image:ZhangLab_2 2007-11-26 13hr 17min-2-.png]] <br> | ||
This could have been a rather good gel if the focus of the gel imaging machine could be adjusted to be either further away of closer to the level of samples. Even though the | |||
[[Image:ZhangLab_2 2007-11-26 14hr 29min.png]]<br> | [[Image:ZhangLab_2 2007-11-26 14hr 29min.png]]<br> | ||
[[Image:ZhangLab_2 2007-11-26 14hr 36min.png]] | [[Image:ZhangLab_2 2007-11-26 14hr 36min.png]] |
Revision as of 08:27, 2 December 2007
Each well contain total of 5 ul of mixture of reagents.
Summary of volumes of Reagents used:
' | well 1 | well 2 | well 3 | well 4 | well 5 | well 6 | well 7 | well 8 | |
Low Mass bp Ladder | 3 ul | 0 ul | 0 ul | 0 ul | 0 ul | 0 ul | 0 ul | 0 ul | |
100 bp Ladder | 0 ul | 3 ul | 0 ul | 0 ul | 0 ul | 0 ul | 0 ul | 0 ul | |
TBE buffer | 1 ul | 1 ul | 0 ul | 0 ul | 0 ul | 0 ul | 0 ul | 0 ul | |
Novex 5x TBE loading dye | 1 ul | 1 ul | 1 ul | 1 ul | 1 ul | 1 ul | 1 ul | 1 ul | |
post-PCR sample | 0 ul | 0 ul | 4 ul | 4 ul | 4 ul | 4 ul | 4 ul | 4 ul | |
Results:
File:ZhangLab 2 2007-11-26 13hr 17min-2-.png
This could have been a rather good gel if the focus of the gel imaging machine could be adjusted to be either further away of closer to the level of samples. Even though the
File:ZhangLab 2 2007-11-26 14hr 29min.png
File:ZhangLab 2 2007-11-26 14hr 36min.png