Daniel:Notebook/ComboLock/2016-6-1: Difference between revisions

From ZhangLabWiki
Jump to navigation Jump to search
>Djacobse
(Created page with "=Antibody Hybridization Test= Back to Calendar Time to test out the antibody binding protocol like I did with [[Daniel:Notebook/ComboLock/2016-...")
 
>Djacobse
No edit summary
Line 5: Line 5:
Time to test out the antibody binding protocol like I did with [[Daniel:Notebook/ComboLock/2016-5-23|C-probes]]
Time to test out the antibody binding protocol like I did with [[Daniel:Notebook/ComboLock/2016-5-23|C-probes]]


==Protocol==
Didn't actually have time to run this today.  See [[Daniel:Notebook/ComboLock/2016-6-1|tomorrow]].
 
<ol>
<li>Mix PLA Buffer</li>
{| class="wikitable" <hiddentext>generated with [[:de:Wikipedia:Helferlein/VBA-Macro for EXCEL tableconversion]] V1.8</hiddentext>
|- style="background-color:#D8E4BC;font-size:12pt" align="center"
| width="118" height="30" | Reagent
| width="101" | Stock Conc.
| width="65" | Final Conc.
| width="65" | Dilution
| width="88" | Volume for 1 mL solution (uL)
 
|- style="font-size:12pt"
| height="15"  valign="bottom" | BSA*
| align="center" valign="bottom" | 50 mg/mL (5%)
| align="center" align="center" valign="bottom" | 0.10%
| align="center" align="center" valign="bottom" | 50
| align="center" align="center" valign="bottom" | 20
 
|- style="background-color:#D9D9D9;font-size:12pt"
| height="15"  valign="bottom" | Tween 20
| align="center" align="center" valign="bottom" | 100%
| align="center" align="center" valign="bottom" | 0.05%
| align="center" align="center" valign="bottom" | 2000
| align="center" align="center" valign="bottom" | 0.5
 
|- style="font-size:12pt"
| height="15"  valign="bottom" | Salmon Sperm DNA
| align="center" valign="bottom" | 10 mg/mL
| align="center" valign="bottom" | 0.1 mg/mL
| align="center" align="center" valign="bottom" | 100
| align="center" align="center" valign="bottom" | 10
 
|- style="background-color:#D9D9D9;font-size:12pt"
| height="15"  valign="bottom" | EDTA
| align="center" valign="bottom" | 500 mM
| align="center" valign="bottom" | 5 mM
| align="center" align="center" valign="bottom" | 100
| align="center" align="center" valign="bottom" | 10
 
|- style="font-size:12pt"
| height="15"  valign="bottom" | PBS
| align="center" valign="bottom" | 10X
| align="center" valign="bottom" | 1X
| align="center" align="center" valign="bottom" | 10
| align="center" align="center" valign="bottom" | 100
 
|- style="background-color:#D9D9D9;font-size:12pt"
| height="15"  valign="bottom" | nfH2O
| align="center" valign="bottom" | --------
| align="center" valign="bottom" | --------
| align="center" valign="bottom" | --------
| align="center" align="center" valign="bottom" | 859.5
 
|}
<li></li>

Revision as of 15:56, 2 June 2016

Antibody Hybridization Test

Back to Calendar

Time to test out the antibody binding protocol like I did with C-probes

Didn't actually have time to run this today. See tomorrow.