Daniel:Notebook/ComboLock/2016-6-25: Difference between revisions
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>Djacobse (Created page with "=Stage 2 Test (Started yesterday)= Back to Calendar ==Protocol== <ol start="7"> <li>Ethanol Precipitat...") |
>Djacobse |
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|- style="font-size:12pt" valign="bottom" | |- style="font-size:12pt" valign="bottom" | ||
| height="15" | Batch2 | | height="15" | Batch2 | ||
| align="center" | | | align="center" | 43.1 | ||
|- style="background-color:#D9D9D9;font-size:12pt" valign="bottom" | |- style="background-color:#D9D9D9;font-size:12pt" valign="bottom" | ||
| height="15" | Batch3 | | height="15" | Batch3 | ||
| align="center" | | | align="center" |11.1 | ||
|- style="font-size:12pt" valign="bottom" | |- style="font-size:12pt" valign="bottom" | ||
| height="15" | Batch5 | | height="15" | Batch5 | ||
| align="center" | | | align="center" | 36.1 | ||
|- style="background-color:#D9D9D9;font-size:12pt" valign="bottom" | |- style="background-color:#D9D9D9;font-size:12pt" valign="bottom" | ||
| height="15" | Batch6 | | height="15" | Batch6 | ||
| align="center" | | | align="center" |18.3 | ||
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Revision as of 20:02, 25 June 2016
Stage 2 Test (Started yesterday)
Protocol
- Ethanol Precipitation
- Centrifuge at 4 C for 25 minutes at 14000 rpm
- Remove supernatant and add 750 uL chilled 75% EtOH
- Centrifuge for 15 minutes at minutes at 4 C at 14000 rpm
- Remove supernatant and dry the pellet in the hood
- Resuspend pellet in 20 uL volume nfH20
- Measure in Nanodrop
- Store DNA at 4C
Nanodrop Results
Batch Source | ng/uL |
Batch2 | 43.1 |
Batch3 | 11.1 |
Batch5 | 36.1 |
Batch6 | 18.3 |