Daniel:Notebook/ComboLock/2016-8-17: Difference between revisions
Jump to navigation
Jump to search
>Djacobse (Created page with "=Positive Control Amplicon Production (Started yesterday)= Back to Calendar ==Protocol== <ol start="7"...") |
>Djacobse |
||
Line 20: | Line 20: | ||
<li>Shred the gel by centrifuging at 14000rpm for 1 min 30 sec</li> | <li>Shred the gel by centrifuging at 14000rpm for 1 min 30 sec</li> | ||
<li>Incubate in 500 uL TBE for 1 hour at 37C with vigorous shaking</li> | <li>Incubate in 500 uL TBE for 1 hour at 37C with vigorous shaking</li> | ||
[[Category:ComboLock]] [[Category:20160805]] |
Revision as of 21:31, 17 August 2016
Positive Control Amplicon Production (Started yesterday)
Protocol
- Size select gel
- Mix 30 uL TBE, 6 uL loading dye, and 4 uL sample for C1-C2 and C3-C2
- Mix 15 uL TBE, 3 uL loading dye, and 2 uL ladder
- Add 20 uL to each lane (2 lanes for C1-C2 and C3-C2)
- Run the gel at 250V for 25 min
- Stain with 3 uL SYBR gold for 3 min
- Image, and extract bands between 100bp and 125 bp; collect the gel bands in the same tube
- Shred the gel by centrifuging at 14000rpm for 1 min 30 sec
- Incubate in 500 uL TBE for 1 hour at 37C with vigorous shaking