Daniel:Notebook/ComboLock/2016-9-3: Difference between revisions
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>Djacobse (Created page with "=X3 Dilution Test (Started Monday)= Back to Calendar ==Protocol (from [[Daniel:Notebook/ComboLock/2016-...") |
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===Nanodrop Results=== | ===Nanodrop Results=== | ||
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Since these results aren't great, I'm going to try again with size select on [[Daniel:Notebook/ComboLock/2016-9-5|Monday]]. | |||
[[Category:Combolock]] [[Category:20160829]] | [[Category:Combolock]] [[Category:20160829]] |
Revision as of 21:58, 5 September 2016
X3 Dilution Test (Started Monday)
Protocol (from yesterday)
- Ethanol Precipitation
- Pellet by centrifuging for 25 minutes at 12000rpm at 4C
- Remove supernatant and add 750 uL chilled 70% EtOH
- Centrifuge for 12 minutes at 12000rpm at 4C
- Remove supernatant and spin in vacuum centrifuge for 10 minutes
- Add 20 uL nfH2O and measure in nanodrop
Nanodrop Results
Sample | ng/uL |
Sample A | 2.5 |
Sample B | 5.9 |
Since these results aren't great, I'm going to try again with size select on Monday.