Daniel:Notebook/ComboLock/2016-9-6: Difference between revisions
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{| class="wikitable" <hiddentext>generated with [[:de:Wikipedia:Helferlein/VBA-Macro for EXCEL tableconversion]] V1.8</hiddentext> | {| class="wikitable" <hiddentext>generated with [[:de:Wikipedia:Helferlein/VBA-Macro for EXCEL tableconversion]] V1.8</hiddentext> | ||
|- style="background-color:#DDD9C4;font-size:12pt;font-weight:bold" align="center" | |- style="background-color:#DDD9C4;font-size:12pt;font-weight:bold" align="center" | ||
| width=" | | width="100" height="30" | Sample | ||
| width="65" | ng/uL | | width="65" | ng/uL | ||
| width="65" | uL added | | width="65" | uL added | ||
| width=" | | width="100" | final mass (ng) | ||
|- style="font-size:12pt" | |- style="font-size:12pt" |
Revision as of 16:16, 9 September 2016
X3 Dilution Test (8/29/2016)
Protocol
- Ethanol Precipitation
- Pellet by centrifuging for 25 minutes at 12000rpm at 4C
- Remove supernatant and add 750 uL chilled 70% EtOH
- Centrifuge for 12 minutes at 12000rpm at 4C
- Remove supernatant and spin in vacuum centrifuge for 10 minutes
- Add 20 uL nfH2O and measure in nanodrop
- Combined 15 uL from each of the two tubes above
- Stored at 4C in upcoming libraries box
Nanodrop Results
Sample | ng/uL |
Sample A | 31 |
Sample B | 19.3 |
Sequencing Library
Sample | ng/uL | uL added | final mass (ng) |
9-6 Sample A | 31 | 15 | 465 |
9-6 Sample B | 19.3 | 15 | 289.5 |