Daniel:Notebook/ComboLock/2017-1-26: Difference between revisions

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<gallery perrow=2 heights=300px widths=300px>
<gallery perrow=2 heights=300px widths=300px>
File:|qPCR curve
File:20170126-qPCR-RCATest-Epicentre.png|qPCR curve
File:2017-01-26-RCATest-Epicentre.png|Gel image
File:2017-01-26-RCATest-Epicentre.png|Gel image
</gallery>
</gallery>

Revision as of 21:03, 26 January 2017

RCA Test (Started Monday)

Back to Calendar

Epicentre Test (Started Yesterday)

Sample Matrix

  1. qPCR
    1. Make 5.2X qPCR master mix according to following recipe
      1. 109.2 uL nfH2o
      2. 5.2 uL AmpF</li
      3. 130 uL 2X Kapa SYBR Fast Master Mix
      4. Add 47 uL master mix to each well
      5. Add 2 uL sample and 1 uL appropriate Reverse Primer (1 uM) according to table
      6. qPCR Cycles
        1. 95C 3 min
        2. 95C 3 sec
        3. 55C 30 sec
        4. 72C 20 sec
        5. plate read
        6. goto b x24
        7. 72C 2 min
        8. 16C hold
    2. TBE Gel
      1. Mix 64 uL TBE, 16 uL 6x loading dye
      2. Aliquot 10 uL per sample/ladder lane onto parafilm
      3. Add 2 uL of sample or ladder to correct drop
      4. Load 10 uL in to well
      5. Run gel for 23 minutes at 230V
      6. Open gel and stain with 3 uL SYBR Gold for 3 minutes
      7. Rinse gel and image in gel doc

    Results