Daniel:Notebook/ComboLock/2017-2-3: Difference between revisions

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(Created page with "=Version 2 Oligos Round 2 (Started yesterday)= Back to Calendar ==Protocol-Part 4==")
 
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[[Daniel:Notebook/ComboLock|Back to Calendar]]
[[Daniel:Notebook/ComboLock|Back to Calendar]]


==Protocol-Part 4==
==Protocol-Part 4-RCA==
 
<ol start="8">
<li>Exonuclease Digestion</li>
<ol type="A">
<li>Mix 15 uL Exo I (20U/uL) and 3 uL ExoIII (100U/uL)</li>
<li>Add 2 uL to each sample, mix by swirling pipette tip</li>
<li>Incubate for 1 hour at 37C</li>
<li>Heat kill by incubating for 5 min at 95C</li>
</ol>
<li>Rolling Circle Amplification</li>
<ol type="A">
<li>Prepare 7X Master mix according to table below</li>
 
 
<li>Add 15 uL master mix to each tube</li>
<li>Add sample to new reaction tubes</li>
<li>Incubate at 37C for 3 hours</li>
<li>Incubate at 65C for 10 minutes;Hold at 10C until next step</li></ol>

Revision as of 17:14, 3 February 2017

Version 2 Oligos Round 2 (Started yesterday)

Back to Calendar

Protocol-Part 4-RCA

  1. Exonuclease Digestion
    1. Mix 15 uL Exo I (20U/uL) and 3 uL ExoIII (100U/uL)
    2. Add 2 uL to each sample, mix by swirling pipette tip
    3. Incubate for 1 hour at 37C
    4. Heat kill by incubating for 5 min at 95C
  2. Rolling Circle Amplification
    1. Prepare 7X Master mix according to table below
    2. Add 15 uL master mix to each tube
    3. Add sample to new reaction tubes
    4. Incubate at 37C for 3 hours
    5. Incubate at 65C for 10 minutes;Hold at 10C until next step