Daniel:Notebook/ComboLock/2017-2-13: Difference between revisions
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[[Daniel:Notebook/ComboLock|Back to Calendar]] | [[Daniel:Notebook/ComboLock|Back to Calendar]] | ||
==Data Analysis== | |||
<ol> | |||
<li>Data was compiled into a single chart</li> | |||
<li>For each of the three potential targets (template,C1 probe, and C2 probe):</li> | |||
<ol type="A"> | |||
<li>Values were averaged across the two technical replicates according to [[Daniel:Notebook/ComboLock/20170210#Plate Layouts|plate specs]]</li> | |||
<li>Averaged CT values were subtracted from the CT value of the NTC corresponding to that plate and target</li> | |||
<li>If the value of the differential was <0 the value was set to 0</li> | |||
</ol></ol> | |||
Results are plotted below for each target separately. | |||
==Results== | ==Results== |
Revision as of 23:05, 13 February 2017
Beads Binding Test (Started Thursday 2-9)
Data Analysis
- Data was compiled into a single chart
- For each of the three potential targets (template,C1 probe, and C2 probe):
- Values were averaged across the two technical replicates according to plate specs
- Averaged CT values were subtracted from the CT value of the NTC corresponding to that plate and target
- If the value of the differential was <0 the value was set to 0
Results are plotted below for each target separately.
Results
- BeadsExperiment-20170210-Template-Annotated.png
Elution curves-template
- BeadsExperiment-20170210-C1Averages-Annotated.png
Elution curves-C1 probe
- BeadsExperiment-20170210-C2Averages-Annotated.png
Elution curves-C2 probe