Daniel:Notebook/ComboLock/2017-3-9: Difference between revisions
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>Djacobse (Created page with "=Bolt Test (Started Yesterday)= Back to Calendar ==Protocol== ol start="3" <li>Bolt/Latch Hyb...") |
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<li>Bolt/Latch Hybridization</li> | <li>Bolt/Latch Hybridization</li> | ||
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Revision as of 00:59, 9 March 2017
Bolt Test (Started Yesterday)
Protocol
- Bolt/Latch Hybridization
- Wash twice with 100 uL wash buffer
- Elution
- Add 50 uL wash buffer to each sample
- Cook at 95C for 15 minutes
- qPCR
- Make master mixes according to following recipes
- Add 48 uL master mix to each well
- Add 2 uL sample according to plate layout File:PlateLayout-20170309-BoltTest.png
Single Rxn | MMC1 | MMC2 | MMP | |
Primer Pair | NA | p24/p4RC | p6/p12RC | p24/p12RC |
uL Fwd Primer (10 uM) | 1 | 10.2 | 10.2 | 10.2 |
uL Rev Primer (10 uM) | 1 | 10.2 | 10.2 | 10.2 |
uL nfH2O | 21 | 214.2 | 214.2 | 214.2 |
uL Kapa SYBR Fast | 25 | 255 | 255 | 255 |
Total | 48 | 489.6 | 489.6 | 489.6 |