Daniel:Notebook/ComboLock/2017-3-29: Difference between revisions
Jump to navigation
Jump to search
>Djacobse |
>Djacobse |
||
Line 8: | Line 8: | ||
<ol> | <ol> | ||
<li>Bglii digestion</li> | |||
<ol type="A"> | |||
<li>Make the following reactions; '''Do not add Bglii yet'''</li> | <li>Make the following reactions; '''Do not add Bglii yet'''</li> | ||
Revision as of 17:40, 29 March 2017
RCA Test (Started March 24)
Bglii Digestion
Testing bglii digestion of RCA product. No qPCR to start with (RCA should be enough)
- Bglii digestion
- Make the following reactions; Do not add Bglii yet
- Use the following thermocycler program
- 5 min 95C
- Ramp to 50C at 0.2C/s
- 10 min 50C
- 2 hr 37C; When this cycle starts add 1 uL Bglii
- Heat kill with 65C for 20 min
Reagent | Single Rxn Vol | Master Mix (30.2X) |
RCA Rxn | 2 | 0 |
Bglii Cutter Primer + (100 uM) | 2 | 60.4 |
10X Buffer 3.1 | 2 | 60.4 |
Bglii | 1 | 0 |
nfH2O | 13 | 392.6 |
Total | 20 | 513.4 |