Daniel:Notebook/ComboLock/2017-4-18: Difference between revisions

From ZhangLabWiki
Jump to navigation Jump to search
>Djacobse
(Created page with "=Circularization Test (Started Jan 23, 2017)= Back to Calendar ==Sequencing Results== These just show ...")
 
>Djacobse
 
Line 13: Line 13:
{| class="wikitable" <hiddentext>generated with [[:de:Wikipedia:Helferlein/VBA-Macro for EXCEL tableconversion]] V1.8</hiddentext>
{| class="wikitable" <hiddentext>generated with [[:de:Wikipedia:Helferlein/VBA-Macro for EXCEL tableconversion]] V1.8</hiddentext>
|- style="font-size:12pt"  valign="bottom"
|- style="font-size:12pt"  valign="bottom"
| width="144" height="45" | M00159:97:000000000-B4K7F:1:1101:11875:18466
| width="144" height="105" | M00159:97:000000000-B4K7F:1:1101:11875:18466
| width="263" | ACTGTATTGTATTTTTTTTTTTTAATGATACGGCGACCACCGAGATCTACACCACTCTCAGATGTTATCGAGGTCCGACGATGTCAGACCGGGCCCACGGG
| width="263" | ACTGTATTGTATTTTTTTTTTTTAATGATACGGCGACCACCGAGATCTACACCACTCTCAGATGTTATCGAGGTCCGACGATGTCAGACCGGGCCCACGGG
| width="108" | ACTGTATTGTA
| width="108" | ACTGTATTGTA

Latest revision as of 00:46, 19 April 2017

Circularization Test (Started Jan 23, 2017)[edit]

Back to Calendar

Sequencing Results[edit]

These just show another poorly hybridized product. The aligned bam files can be found in genome miner at:

/media/NAS3_volume2/Dan/combolock/miseq_20170414

Most of the reads have ~50bp matching, which is almost always the AmpF signal. I also discovered something upon close examination, shown in the read below. There is a small overlap at the final bases of LinkerR and first few bases of primer24RC. Apparently at high enough concentrations this causes trouble.

M00159:97:000000000-B4K7F:1:1101:11875:18466 ACTGTATTGTATTTTTTTTTTTTAATGATACGGCGACCACCGAGATCTACACCACTCTCAGATGTTATCGAGGTCCGACGATGTCAGACCGGGCCCACGGG ACTGTATTGTA TTTTTTTTTTTT AATGATACGGCGACCACCGA GATCTACACCACTCTCAGAT GTTATCGAGGTCCGACGATGTCAGAC CGGGCCCACGGG