Daniel:Notebook/ComboLock/2017-4-18: Difference between revisions
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>Djacobse (Created page with "=Circularization Test (Started Jan 23, 2017)= Back to Calendar ==Sequencing Results== These just show ...") |
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{| class="wikitable" <hiddentext>generated with [[:de:Wikipedia:Helferlein/VBA-Macro for EXCEL tableconversion]] V1.8</hiddentext> | {| class="wikitable" <hiddentext>generated with [[:de:Wikipedia:Helferlein/VBA-Macro for EXCEL tableconversion]] V1.8</hiddentext> | ||
|- style="font-size:12pt" valign="bottom" | |- style="font-size:12pt" valign="bottom" | ||
| width="144" height=" | | width="144" height="105" | M00159:97:000000000-B4K7F:1:1101:11875:18466 | ||
| width="263" | ACTGTATTGTATTTTTTTTTTTTAATGATACGGCGACCACCGAGATCTACACCACTCTCAGATGTTATCGAGGTCCGACGATGTCAGACCGGGCCCACGGG | | width="263" | ACTGTATTGTATTTTTTTTTTTTAATGATACGGCGACCACCGAGATCTACACCACTCTCAGATGTTATCGAGGTCCGACGATGTCAGACCGGGCCCACGGG | ||
| width="108" | ACTGTATTGTA | | width="108" | ACTGTATTGTA |
Latest revision as of 00:46, 19 April 2017
Circularization Test (Started Jan 23, 2017)[edit]
Sequencing Results[edit]
These just show another poorly hybridized product. The aligned bam files can be found in genome miner at:
/media/NAS3_volume2/Dan/combolock/miseq_20170414
Most of the reads have ~50bp matching, which is almost always the AmpF signal. I also discovered something upon close examination, shown in the read below. There is a small overlap at the final bases of LinkerR and first few bases of primer24RC. Apparently at high enough concentrations this causes trouble.
M00159:97:000000000-B4K7F:1:1101:11875:18466 | ACTGTATTGTATTTTTTTTTTTTAATGATACGGCGACCACCGAGATCTACACCACTCTCAGATGTTATCGAGGTCCGACGATGTCAGACCGGGCCCACGGG | ACTGTATTGTA | TTTTTTTTTTTT | AATGATACGGCGACCACCGA | GATCTACACCACTCTCAGAT | GTTATCGAGGTCCGACGATGTCAGAC | CGGGCCCACGGG |