Daniel:Notebook/PosSequencing/2017-4-27: Difference between revisions

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(Created page with "=Emulsion PCR (Started Yesterday)= Back to Calendar ==Protocol Part 3-Hybridization and Imaging...")
 
>Djacobse
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<li>Put suspension on magnet</li>
<li>Put suspension on magnet</li>
<li>Resuspend beads in 25 uL 4X SSC buffer</li>
<li>Resuspend beads in 25 uL 4X SSC buffer</li>
<li>Add 25 uL fluorescent probe in 60% formamide</li>
<li>Wash three times in 2X SSC</li>
</ol>
</ol>
<li>Imaging</li>
<li>Imaging</li>

Revision as of 00:16, 27 April 2017

Emulsion PCR (Started Yesterday)

Back to Calendar

Protocol Part 3-Hybridization and Imaging

  1. Fluorescent Oligo Binding
    1. Put suspension on magnet
    2. Resuspend beads in 25 uL 4X SSC buffer
    3. Add 25 uL fluorescent probe in 60% formamide
    4. Wash three times in 2X SSC
  2. Imaging