Daniel:Notebook/PosSequencing/2017-4-27: Difference between revisions
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>Djacobse (Created page with "=Emulsion PCR (Started Yesterday)= Back to Calendar ==Protocol Part 3-Hybridization and Imaging...") |
>Djacobse |
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<li>Put suspension on magnet</li> | <li>Put suspension on magnet</li> | ||
<li>Resuspend beads in 25 uL 4X SSC buffer</li> | <li>Resuspend beads in 25 uL 4X SSC buffer</li> | ||
<li>Add 25 uL fluorescent probe in 60% formamide</li> | |||
<li>Wash three times in 2X SSC</li> | |||
</ol> | </ol> | ||
<li>Imaging</li> | <li>Imaging</li> |
Revision as of 00:16, 27 April 2017
Emulsion PCR (Started Yesterday)
Protocol Part 3-Hybridization and Imaging
- Fluorescent Oligo Binding
- Put suspension on magnet
- Resuspend beads in 25 uL 4X SSC buffer
- Add 25 uL fluorescent probe in 60% formamide
- Wash three times in 2X SSC
- Imaging