Daniel:Notebook/PosSequencing/2017-4-27: Difference between revisions

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==Protocol Part 3-Hybridization and Imaging==
==Protocol Part 3-Hybridization and Imaging==
Probe hybridization and washes based on [[Matt:LabNotes/2015-3-12|Matt's experiment]]. Calculations for dye binding:
{{math|''α''}}


<ol start="7">
<ol start="7">
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<li>Resuspend beads in 25 uL 4X SSC buffer</li>
<li>Resuspend beads in 25 uL 4X SSC buffer</li>
<li>Add 25 uL fluorescent probe in 60% formamide</li>
<li>Add 25 uL fluorescent probe in 60% formamide</li>
<li>Incubate at room temp in the dark for 30 min</li>
<li>Wash three times in 2X SSC</li>
<li>Wash three times in 2X SSC</li>
</ol>
</ol>

Revision as of 15:48, 27 April 2017

Emulsion PCR (Started Yesterday)

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Protocol Part 3-Hybridization and Imaging

Probe hybridization and washes based on Matt's experiment. Calculations for dye binding:

Template:Math


  1. Fluorescent Oligo Binding
    1. Put suspension on magnet
    2. Resuspend beads in 25 uL 4X SSC buffer
    3. Add 25 uL fluorescent probe in 60% formamide
    4. Incubate at room temp in the dark for 30 min
    5. Wash three times in 2X SSC
  2. Imaging