Daniel:Protocols/AmiconUltra: Difference between revisions

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(Created page with "=Amicon Ultra Centrifugal Filters= Back to Main [https://www.emdmillipore.com/US/en/product/Amicon-Ultra-0.5%C2%A0mL-Centrifugal-Filters-for-DNA-and-Prot...")
 
>Djacobse
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[[Image:CentrifugalFilters.png|400px]]
[[Image:CentrifugalFilters.png|400px]]


==Protocol==
==Protocol-Filtration==
 
<ol>
<li>Add X uL sample and buffer to 500 uL total volume</li>
<li>Spin down at 14000 x g for appropriate time (15 minutes for 22 uL volume)</li>
<li>Discard flow through tube and get a new one</li>
<li>Invert filter inside flow through tube and spin down at 14000xg for 1 minute</li>
<li>Alternately, extract sample using pipette</li>
</ol>
 
==Protocol-Dialysis==
 
<ol>
<li><li>Add X uL sample and new buffer to 500 uL total volume</li>
<li>Spin down at 14000 x g for appropriate time (15 minutes for 22 uL volume)</li>
<li>Discard flow through and add 500 uL new buffer</li>
<li>Repeat steps 2 and 3 as many times as desired</li></ol>

Revision as of 21:43, 13 May 2017

Amicon Ultra Centrifugal Filters

Back to Main

Amicon Order Page/Information

Amicon Ultra Centrifugal filters are microcentrifuge filters that allow you to purify and buffer exchange various MW cutoff biomacromolecules. They use a MW cutoff filter, say 10 kDa, to allow dialysate through while not passing the biomacromolecules. They can be used for buffer exchange via the following general protocol:

File:CentrifugalFilters.png

Protocol-Filtration

  1. Add X uL sample and buffer to 500 uL total volume
  2. Spin down at 14000 x g for appropriate time (15 minutes for 22 uL volume)
  3. Discard flow through tube and get a new one
  4. Invert filter inside flow through tube and spin down at 14000xg for 1 minute
  5. Alternately, extract sample using pipette

Protocol-Dialysis

  1. Add X uL sample and new buffer to 500 uL total volume
  2. Spin down at 14000 x g for appropriate time (15 minutes for 22 uL volume)
  3. Discard flow through and add 500 uL new buffer
  4. Repeat steps 2 and 3 as many times as desired