Daniel:Notebook/PosSequencing/2017-7-12: Difference between revisions

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(Created page with "=emPCR8 (Started Thursday July 6)= Back to Calendar <ol start="10"> <li>Dye hybridization</li>...")
 
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[[Daniel:Notebook/PosSequencing|Back to Calendar]]
[[Daniel:Notebook/PosSequencing|Back to Calendar]]


==Protocol==


<ol start="10">
<ol start="10">

Revision as of 15:42, 12 July 2017

emPCR8 (Started Thursday July 6)

Back to Calendar

Protocol

  1. Dye hybridization
    1. Mix 20 uL ssDNA (lambda digested) and 20 uL dsDNA (original, non-digested template)
    2. Add 1 uL appropriate (10 uM) dye, A488-dc6 for sample 1 and Cy5-dc6 for sample 3
    3. Incubate in the dark (RT) for 15 minutes
    4. Magnet pulldown, remove supernatant and add 40 uL 2X SSC buffer
    5. Add 15 uL to a slide for analysis
    6. Seal coverslip with nail polish and wait approximately 30 minutes to dry