Kun:LabNotes/CpgSeq/2007-12-9: Difference between revisions
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(New page: ===Exp.l PCR on the captured CpG islands=== *Template: two capturing reactions started at 12/07/2007. *Primer: Amp_F_sol, Amp_R_sol x 1 x 4 te...) |
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H2O: 35.3ul 142ul | H2O: 35.3ul 142ul | ||
94C 3min -> 10 cycles of (94C 45sec -> 58C 2min -> 72C 1min) -> | 94C 3min -> 10 cycles of (94C 45sec -> 58C 2min -> 72C 1min) -> 25 cycles of (94C 45 sec -> 70C 1min) -> 72C 3min -> 15C hold. <br> |
Revision as of 23:49, 9 December 2007
Exp.l PCR on the captured CpG islands
- Template: two capturing reactions started at 12/07/2007.
- Primer: Amp_F_sol, Amp_R_sol
x 1 x 4 template: 5ul JumpStart 10x buffer: 4.5ul 18ul 10mM dNTP: 1ul 4ul 100uM AmpFSol: 0.15ul 0.6ul 100uM AmpRSol: 0.15ul 0.6ul 50X SYBG I: 0.4ul 1.6ul JumpStart Taq: 0.5ul 2ul H2O: 35.3ul 142ul
94C 3min -> 10 cycles of (94C 45sec -> 58C 2min -> 72C 1min) -> 25 cycles of (94C 45 sec -> 70C 1min) -> 72C 3min -> 15C hold.