Kun:LabNotes/CpgSeq/2007-12-9: Difference between revisions

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(New page: ===Exp.l PCR on the captured CpG islands=== *Template: two capturing reactions started at 12/07/2007. *Primer: Amp_F_sol, Amp_R_sol x 1 x 4 te...)
 
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       H2O:                  35.3ul        142ul
       H2O:                  35.3ul        142ul


94C 3min -> 10 cycles of (94C 45sec -> 58C 2min -> 72C 1min) -> 20 cycles of (94C 45 sec -> 70C 1min) -> 72C 3min -> 15C hold. <br>
94C 3min -> 10 cycles of (94C 45sec -> 58C 2min -> 72C 1min) -> 25 cycles of (94C 45 sec -> 70C 1min) -> 72C 3min -> 15C hold. <br>

Revision as of 23:49, 9 December 2007

Exp.l PCR on the captured CpG islands

  • Template: two capturing reactions started at 12/07/2007.
  • Primer: Amp_F_sol, Amp_R_sol
                              x 1         x 4
     template:                5ul         
     JumpStart 10x buffer:  4.5ul         18ul
     10mM dNTP:               1ul          4ul
     100uM AmpFSol:        0.15ul        0.6ul
     100uM AmpRSol:        0.15ul        0.6ul
     50X SYBG I:            0.4ul        1.6ul
     JumpStart Taq:         0.5ul          2ul
     H2O:                  35.3ul        142ul

94C 3min -> 10 cycles of (94C 45sec -> 58C 2min -> 72C 1min) -> 25 cycles of (94C 45 sec -> 70C 1min) -> 72C 3min -> 15C hold.