Kun:LabNotes/ASE/2007-12-14: Difference between revisions

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(New page: ==Exp.1 PCR on the circularized CES22k probes== one reaction 12 reactions Template: 10ul each 10x B...)
 
Line 12: Line 12:
           H2O                  72ul                861ul
           H2O                  72ul                861ul


94C 3min -> 10 cycles of (94C 45sec -> 57C 1min -> 72C 1min) -> 10 cycles of (94C 45 sec -> 72 1min) -> 72C 3min -> 15C hold.
94C 3min -> 8 cycles of (94C 45sec -> 57C 1min -> 72C 1min) -> 10 cycles of (94C 45 sec -> 72 1min) -> 72C 3min -> 15C hold.
 
Took out 1C and 2A, add 4 more cycles on the remaining reactions.

Revision as of 23:37, 14 December 2007

Exp.1 PCR on the circularized CES22k probes

                              one reaction       12 reactions
          Template:             10ul                  each
          10x Buffer:            9ul                 108ul
          25mM MgCl2           5.4ul                64.8ul
          25mM dNTP            1.2ul                13.2ul
          100uM AmpSolV6F2     0.4ul                 4.8ul
          100uM AmpSolV6R2     0.4ul                 4.8ul
          JumpStart Taq          1ul                  12ul
          50X SYBG I           0.8ul                 9.6ul
          H2O                   72ul                 861ul

94C 3min -> 8 cycles of (94C 45sec -> 57C 1min -> 72C 1min) -> 10 cycles of (94C 45 sec -> 72 1min) -> 72C 3min -> 15C hold.

Took out 1C and 2A, add 4 more cycles on the remaining reactions.