AlanFung:Protocol/OliGreen ssDNA Stain: Difference between revisions

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>Alan6017518
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  *20X TE Buffer (Component B)
  *20X TE Buffer (Component B)
  *Oligonucleotide Standard (Component C) 18-base M13 sequence primer,  
  *Oligonucleotide Standard (Component C) 18-base M13 sequence primer,  
with the sequence 5'-TGTAAAACGACGGCCAGT-3'
  with the sequence 5'-TGTAAAACGACGGCCAGT-3'
*Sterile, distilled, DNase-free water
*Sterile, distilled, DNase-free water
*96 well microplates
*96 well microplates

Revision as of 18:14, 28 May 2009

Probe Preparation

Samples & Materials

  • Quant-iT OliGreen ss DNA Assay Kit (Cat. no. O11492)
*Quant-iT OliGreen ss DNA Reagent (Component A), solution indimethylsulfoxide (DMSO)
*20X TE Buffer (Component B)
*Oligonucleotide Standard (Component C) 18-base M13 sequence primer, 
 with the sequence 5'-TGTAAAACGACGGCCAGT-3'
  • Sterile, distilled, DNase-free water
  • 96 well microplates
  • Microplate Reader

Procedure

Allow