AlanFung:Protocol/Genome Analyzer

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Revision as of 13:40, 21 July 2009 by >Alan6017518 (→‎image cycletab)
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Genome Analyzer User Guide

SCS v2.4

  • start up scsv2.4

setting the run parameters

  • click <view>
  • clear box next to show on start up
  • from the data collection screen
  • edit|run parameters|GA Settings / RunBrowser Settings

GA Setting

  • specify the network location for run results
  • set perferences for real time analysis results
  • set settings for auto calibration
*delete images (select this option to delete images from the genome analyzer computer after analysis

Validation strings

  • edit if you are using a customized identification system

Use auto calibration

  • select this option to enable the auto calibration feature
  • refer to page 29 for a description of the auto calibration feature
  • note: if this option is cleared, focus calibration must be performed manually as described in appendix B

Run Browser Settings

  • runbrowzer reports, specify which runbrowser reports you want to show and export
if selected the first cycle is generated by the software
  • enable runbrowser report emails to sens reports to the email addresses specified
  • after you have finished selecting the settings in the run parameters window, click <save & continue>/<cancel & continue>/<reset default configs>

initialize the software

  • click the manual control/setup tab
  • perform any operation to trigger the genome analyzer initialization

run window

recipe tab

  • hover over a stop to show the parameters specific to that step

image cycletab

white tile are queued for imaging
blue tiles haven been imaged
gray tiles are not defined in the current run
right side of the window shows the photographs being taken of the current tile

temperature tab

  • for monitor various run temperatures

manual control/setup window

manual control

Cluster Station