HUES
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HUES Cell Collection
HUES homepage
- HUES homepage[1]
Protocol
- Protocol Media:hues.pdf
Storage
- Location:Dr. Varghese lab LN tank Rack #2 Box "Zhang Lab"
HUES Cell Log
- 1 x HUES-3 p22
- 1 x HUES-8 p19
- 1 x HUES-53 p11
- 1 x HUES-62 p10
- 1 x HUES-64 p12
- 1 x HUES-66 p12
- 2 x PMEFi
Materials and Reagents
Materials / Catalogue no. | Provider | CAT# |
KO‐DMEM | Invitrogen Gibco | CAT#10829 |
DMEM | Invitrogen Gibco | CAT#11965‐092 |
FBS | Valley Biomedical, Inc | CAT#BS‐3033 |
KO‐Serum Replacenment | Invitrogen Gibco | CAT#10828 |
Plasmanate | Bayer | CAT#NDC 0026‐0613‐20 |
bFGF | Invitrogen Gibco | CAT#13256‐029 |
hLIF | Chemicon International | CAT#LIF1010 |
2‐beta‐mercaptoethanol | Gibco | CAT#21985‐023 |
Non‐essential amino acids (NEAA) | Invitrogen Gibco | CAT#11140050 |
(10mM or 100x) | ||
Pen/Strep | Invitrogen Gibco | CAT#15070‐063 |
(5000U/ml Penicillin G sodium and | ||
5000ug/ml Streptomycin sulfate in 0.85% saline) | ||
Glutamax‐I | Invitrogen Gibco | CAT#35050‐061 |
(200mM or 100x) | ||
Mitomycin C | Sigma | CAT#M‐0503 |
0.05% Trypsin/EDTA | Invitrogen Gibco | CAT#25300‐054 |
Dimethyl sulphoxide (DMSO) | Sigma | CAT#D2650 |
Gelatin | Sigma | CAT#G1890 |
Tissue Culture Treated Plates (6 well) | Coring | CAT#3506 |
Hygromycin resistant, pre-treated MEFs | Specialty Media | CAT#PMEF‐H |
Neomycin resistant, pre-treated MEFs | Specialty Media | CAT#PMEF‐N |
Contact Information
HUES Cell Facility Contact Information
- HUES Facility / Melton Laboratory / HHMI
- Harvard University
- Cambridge, MA 02138
- Fax: 617.384.9463
- Please send all HUES cell correspondence to hues@mcb.harvard.edu.
Harvard's Office of Technology Development Contact Information
- Harvard's Office of Technology Development
- 1350 Massachusetts Avenue
- Holyoke Center 727
- Cambridge, MA 02138
- Fax: 617.495.9568
- Please send all correspondence concerning the MTA to Material Transfers: materialtransfer@harvard.edu.