Jie:LabNotes/CpgSeq/2009-8-27

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Revision as of 20:25, 27 August 2009 by >Jie deng
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ASM validation of cell type and individual dependent ASM

primers for cell and individual dependent ASM validation 
I did the PCR using 2x iQ supermix on:

chr12:4541690 on PGP1L and PGP1F;
chr21:32746340 on PGP1L and PGP1F;
chr20:60061824 on PGP3L and PGP9L;
chr20:60403281 on PGP1L and PGP3L; 
rs3746459 on BJ;
                                                         x 9
2x iQ supermix                          50             450          
Primer F (50uM)			 0.5	        0.5 each
Primer R (50uM)			 0.5            0.5 each
sample bis-gDNA (~202ng/ul)             0.1	  	0.1 each
dH2O				         50		450
total				         40ul
96C 3min-> 45x (95C 30sec, MATN4 and DNMT3B at 58C, rs10846023 on 62C 1min, 72C 1min)-> 72C 5min.