Revision as of 18:39, 5 December 2009 by >Alan6017518
qPCR Quantification
PGP4 PGP6 PGP8 PGP9 PGP8D PGP8E Dpn-CV-iF2 Dpn-CV-F2
Setup |
|
|
|
|
Amp Phi-X (1nM) |
PGP6 |
PGP9 |
PGP8E |
Dpn-CV-iF2 (1/10)
|
Amp Phi-X (1nM) |
PGP6 |
PGP9 |
PGP8E |
Dpn-CV-iF2 (1/10)
|
Amp Phi-X (1nM) |
PGP6 |
PGP9 |
PGP8E |
Dpn-CV-iF2 (1/10)
|
Amp Phi-X (1nM) |
PGP6 |
PGP9 |
PGP8E |
Dpn-CV-iF2 (1/10)
|
PGP4 |
PGP8 |
PGP8D |
Dpn-CV-F2 (1/10) |
|
PGP4 |
PGP8 |
PGP8D |
Dpn-CV-F2 (1/10) |
|
PGP4 |
PGP8 |
PGP8D |
Dpn-CV-F2 (1/10) |
|
PGP4 |
PGP8 |
PGP8D |
Dpn-CV-F2 (1/10) |
|
|
|
|
x18 |
*1.1
|
DNA |
2 |
|
|
Syb_FP5 (100uM) |
0.2 |
3.6 |
3.96
|
Syb_RP7 (100uM) |
0.2 |
3.6 |
3.96
|
2X Phusion |
25 |
450 |
495
|
50X SYBR Green I |
0.2 |
3.6 |
3.96
|
H20 |
24 |
432 |
475.2
|
Total |
|
|
982.08
|
|
- Dilute sample, 1ul in 9ul ddh2o
- mix 2ul 1/10 sample with 98ul mix
- aliquot 24ul to each well
98C 30 sec -> 20 cycles of (98C 10sec->64C 20sec->72C 20sec)->72C 5 min