Revision as of 23:09, 5 December 2009 by >Alan6017518
qPCR Quantification
PGP4 PGP6 PGP8 PGP9 PGP8D PGP8E Dpn-CV-iF2 Dpn-CV-F2
Setup |
|
|
|
|
Amp Phi-X (1nM) |
PGP6 |
PGP9 |
PGP8E |
Dpn-CV-iF2 (1/10)
|
Amp Phi-X (1nM) |
PGP6 |
PGP9 |
PGP8E |
Dpn-CV-iF2 (1/10)
|
Amp Phi-X (1nM) |
PGP6 |
PGP9 |
PGP8E |
Dpn-CV-iF2 (1/10)
|
Amp Phi-X (1nM) |
PGP6 |
PGP9 |
PGP8E |
Dpn-CV-iF2 (1/10)
|
PGP4 |
PGP8 |
PGP8D |
Dpn-CV-F2 (1/10) |
|
PGP4 |
PGP8 |
PGP8D |
Dpn-CV-F2 (1/10) |
|
PGP4 |
PGP8 |
PGP8D |
Dpn-CV-F2 (1/10) |
|
PGP4 |
PGP8 |
PGP8D |
Dpn-CV-F2 (1/10) |
|
|
|
|
x18 |
*1.1
|
DNA |
2 |
|
|
Syb_FP5 (100uM) |
0.2 |
3.6 |
3.96
|
Syb_RP7 (100uM) |
0.2 |
3.6 |
3.96
|
2X Phusion |
25 |
450 |
495
|
50X SYBR Green I |
0.2 |
3.6 |
3.96
|
H20 |
24 |
432 |
475.2
|
Total |
|
|
982.08
|
|
- Dilute sample, 1ul in 9ul ddh2o
- mix 2ul 1/10 sample with 98ul mix
- aliquot 24ul to each well
98C 30 sec -> 20 cycles of (98C 10sec->64C 20sec->72C 20sec)->72C 5 min
|
|
|
|
|
Average |
Difference |
2^ |
nM
|
Amp Phi-X (1nM) |
9.833 |
9.899 |
9.819 |
9.990 |
9.89 |
0 |
|
|
PGP4 |
9.471 |
9.522 |
9.562 |
9.706 |
9.57 |
0.32 |
1.25 |
1.25
|
PGP6 |
10.667 |
10.911 |
11.011 |
|
10.86 |
0.97 |
1.96 |
0.51
|
PGP8 |
9.719 |
9.813 |
10.088 |
10.166 |
9.95 |
0.06 |
1.04 |
0.96
|
PGP9 |
9.663 |
9.710 |
9.751 |
|
9.71 |
0.18 |
1.13 |
1.13
|
PGP8D |
5.250 |
5.262 |
5.287 |
|
5.27 |
4.62 |
24.65 |
24.65
|
PGP8E |
5.067 |
5.143 |
5.168 |
|
5.13 |
4.76 |
27.17 |
27.17
|
Dpn-CV-F2 (1/10) |
8.303 |
8.403 |
8.663 |
8.666 |
8.51 |
1.38 |
2.6 |
26
|
Dpn-CV-iF2 (1/10) |
9.824 |
9.962 |
10.000 |
|
9.93 |
0.04 |
1.03 |
9.74
|
|