AlanFung:LabNotes/CTCF/2010-1-15
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Quantify Bisulfite Converted Samples with Qubit ssDNA Kit
On 50ng, 10ng, 5ng, 1ng, 0.5ng, 0.1ng & 0.05ng P21-200BP
7 samples Prepare 7 Qubit Assay tubes Prepare 7*200ul diluted dye=1400ul Mix 7ul dye with 1393ul buffer
Mix 2ul samples with 98ul diluted dye vortex spin down and wait for 2 mins Result
Start (ng) | Sample Concentration ng/ul | Volume | Amount Recovered (ng) | Yield % |
50 | 1.7 | 20 | 34 | 68 |
10 | 0.25 | 20 | 5 | 50 |
5 | 0.13 | 20 | 2.6 | 52 |
1 | 0.26 | 20 | 5.2 | 520 |
0.5 | N/A | 20 | ||
0.1 | N/A | 20 | ||
0.05 | N/A | 20 | ||