- Received 12 BSC sample derived from amplified and un-amplified circulating tumor DNA from patients from Illumina
Sample_ID
Sample_wel
BSC input (ng)
Preamp?
Conc. (ng/ul)
Volume (ul)
BSC_10ng_restored_1-1
A1
10
Yes
569.3
10
BSC_10ng_restored_1-2
A2
10
Yes
577.6
10
BSC_1ng_restored_1-1
A3
1
Yes
427.9
10
BSC_1ng_restored_1-2
A4
1
Yes
437.0
10
BSC_10ng_restored_2-1
A5
10
Yes
546.8
10
BSC_10ng_restored_2-2
A6
10
Yes
494.3
10
BSC_1ng_restored_2-1
A7
1
Yes
338.8
10
BSC_1ng_restored_2-2
A8
1
Yes
358.0
10
BSC_80ng_restored
A9
80
Yes
566.7
10
BSC_30ng_restored
A10
30
Yes
615.3
10
BSC_80ng
B1
80
No
8.0
10
BSC_30ng_1
B2
30
No
3.0
10
BSC_30ng_2
B3
30
No
3.0
10
The concentration of these samples were changed because I added more TE buffer and re-quantified concentration by Qubit quantification
April 14, 2014 (8 additional amplified DNA samples from Illumina)
Received 8 additional amplified DNA samples from Xuyu Cai
No information of sample was provide only 5ul for each one and concentration ~200ng/ul
I added more H2O to bring volume to 12ul, transfered samples to 8-tube strip with individual cap, and re-quantified concentration with Qubit ssDNA HS assay
Qubit dsDNA assay HS results
Sample
Conc. in the Qubit
Unit
uL used
Dilution
Sample conc. (ng/ul)
Total volume
Total amount (ng)
#1
173
ng/mL
1
200
34.6
12
415.2
#2
152
ng/mL
1
200
30.4
12
364.8
#3
199
ng/mL
1
200
39.9
12
478.8
#4
207
ng/mL
1
200
41.4
12
496.8
#5
108
ng/mL
1
200
21.6
12
259.2
#6
154
ng/mL
1
200
30.9
12
370.8
#7
181
ng/mL
1
200
36.1
12
433.2
#8
172
ng/mL
1
200
34.4
12
412.8
5-06-2014
Received ~30ul of polymerase with property similar to Amplitaq Stoffel fragment. Conc. 3U/ul. She is not allowed to provide any further info. of this enzyme.
May 22, 2014 (Plasma samples from normal samples with no cancer history
Received 30 plasma samples 1mL each from Kang Zhang's lab
All samples were collected in 2014
Zhang Id
phenotype_category
sex
date_of_birth
Age
ethnicity
15849001
Normal - Other
M
2/13/1946
68
Caucasian - European
15898001
Normal Control
F
1/21/1983
31
Caucasian - European
16188001
Normal Control
M
2/21/1954
60
Caucasian - European
16191001
Normal Control
M
9/25/1964
50
Caucasian - European
16296003
Normal Control
F
8/8/1976
38
Caucasian - European
16345002
Normal Control
F
3/31/1986
28
Caucasian - European
16405003
Normal Control
F
8/9/1960
54
Caucasian - European
16408001
Normal Control
M
3/12/1944
70
Caucasian - European
16409002
Normal Control
F
10/30/1943
71
Caucasian - European
16409003
Normal Control
M
8/10/1971
43
Caucasian - European
16416001
Normal Control
F
11/4/1949
65
Caucasian - European
16461001
Normal Control
F
1/31/1936
78
Caucasian - European
16508001
Normal Control
M
8/13/1973
41
Caucasian - European
16509001
Normal Control
F
3/8/1964
50
Caucasian - European
16510002
Normal Control
M
8/26/1957
57
Caucasian - European
16512001
Normal Control
M
6/1/1982
32
Caucasian - European
16517001
Normal Control
M
4/3/1949
65
Caucasian - European
16518001
Normal Control
M
1/18/1955
59
Caucasian - European
16519001
Normal Control
F
6/13/1976
38
Caucasian - European
16520001
Normal Control
M
12/30/1954
60
Caucasian - European
16523001
Normal Control
M
7/8/1955
59
Caucasian - European
16524001
Normal Control
F
1/11/1953
61
Caucasian - European
16534001
Normal Control
F
3/4/1992
22
Caucasian - European
16553001
Normal Control
F
3/11/1938
76
Caucasian - European
16557001
Normal Control
M
6/8/1948
66
Caucasian - European
16561001
Normal Control
F
8/28/1972
42
Caucasian - European
16562001
Normal Control
M
3/8/1983
31
Caucasian - European
16563001
Normal Control
M
1/31/1954
60
Caucasian - European
16564001
Normal Control
F
2/23/1986
28
Caucasian - European
16565001
Normal Control
F
10/22/1968
46
Caucasian - European
November5, 2010 (Mouse gDNA from La Spada's lab, UCSD)
received 8 gDNA samples from Paula Ladd
Samples
Sample ID
concentration (ng/ul)
volume (ul)
1. wild-type cortex
WT-CTX
2000.0
3.8
2. wild-type cerebellum
WT-CBL
100.0
8.2
3. wild-type brain stem
WT-BS
600.0
8.5
4. wild-type retina
WT-RET
1800.0
4.0
5. transgenic cortex
TG-CTX
250.0
17.0
6. transgenic cerebellum
TG-CBL
400.0
14.0
7. transgenic brain stem
TG-BS
1000.0
6.5
8. transgenic retina
TG-RET
2000.0
8.0
Note: WT: wild-type, TG: transgenic
Samples were diluted to 200ng/ul based on original concentration from Paula and confirmed by Nanodrop
Samples
Sample ID
total volume (ul) after diluted
concentration(ng/ul)
1. wild-type cortex
WT-CTX
25.0
223.2
2. wild-type cerebellum
WT-CBL
25.0
663.5
3. wild-type brain stem
WT-BS
25.0
86.0
4. wild-type retina
WT-RET
25.0
163.7
5. transgenic cortex
TG-CTX
20.0
219.2
6. transgenic cerebellum
TG-CBL
25.0
109.4
7. transgenic brain stem
TG-BS
25.0
103.0
8. transgenic retina
TG-RET
25.0
126.6
labnote: 2010_11_08
December 7, 2010 (Sarah's African gDNA, UPenn )
received 48 methylome samples from Tishkoff's lab delivered by UPS
William R Beggs, Jr. Tishkoff Lab - Dept of Genetics, University of Pennsylvania 430 Clinical Research Building 415 Curie Blvd Philadelphia, PA 19104-6145
LabID
1M sample #
Well
Volume ul
Conc ng/ul approx
CAPB016
A01
50
100
CAPB046
B01
50
100
CAPB056a
C01
50
100
CAPL056
D01
50
100
CAPM007
E01
50
100
TZHZ018A
F01
50
100
TZHZ033B
G01
50
100
TZHZ075A
H01
50
100
TZHZ214
A02
50
100
TZHZ221B
B02
50
100
TZSW067
C02
50
100
TZSW128B
D02
50
100
TZSW131A
E02
50
100
TZSW132B
F02
50
100
TZSW135B
G02
50
100
CAMF013A
H02
50
100
CAFU043
A03
50
100
CAFU042
B03
50
100
CAFU028
C03
50
100
CAMF022A
D03
50
100
CAPB043
E03
50
100
CAPL049
F03
50
100
CAPM001A
G03
50
100
CAPL036
H03
50
100
KEBR007A
A04
50
100
KEBR028A
B04
50
100
KEBR042B
C04
50
100
KEBR040A
D04
50
100
KEBR061A
E04
50
100
KEPK003A
F04
50
100
KEPK006A
G04
50
100
KEPK007A
H04
50
100
KEPK010A
A05
50
100
KEPK016A
B05
50
100
CAPM047
son
C05
50
100
CAPM003A
parent
D05
50
100
CAPM004A
parent
E05
50
100
CAPM056A
daughter
F05
50
100
ETAM042
G05
50
100
ETAM071
H05
50
100
ETAM077
A06
50
100
ETAM058
B06
50
100
ETAM065
C06
50
100
ETSB008
D06
50
100
ETSB027
E06
50
100
ETSB031
F06
50
100
ETSB035
G06
50
100
ETSB036
H06
50
100
aliquot 21ul out of 50ul samples into another 96-well plate
Note: Since the capture of the GA plate1 failed, and I needed to repeated the experiment, Ling brought the new plate (called GA plate1-2) with the same samples to me and all concentration were measured again by Nanodrop.
Note: Since I thought that they all are brain tissue samples, some labeling of library name or images used brain instead of blood. To prevent confusing, every labeling should be changed.
August25, 2011, gDNA from fibroblast cells that are the somatic line of the three single nonsynonymous mutation iPS on the record of July29, 2011
Received 2 gDNA samples from fibroblast cells that are the somatic lines of the three single nonsynonymous mutation iPS from Alice. Both of them were from the first elution in DNA purification