Noi:Probe preparation data/Probes Prep
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Summary of DMR220k probe set production
Probe set | No. of rxn | Date of production | Enzyme | Conc. (ng/ul) | Vol. (ul) | Yields (ug) | for DNA capture |
S1 | 96 rxn | 2010_10_24 | Kapa | 26.20 | 250.00 | 6.50 | diluted to 2ng/ul vol 100ul |
S2 | 96 rxn | 2010_10_24 | Kapa | 30.10 | 250.00 | 7.50 | diluted to 2ng/ul vol 100ul |
S3 | 96 rxn | 2010_10_24 | Kapa | 23.20 | 250.00 | 5.80 | |
S4 | 96 rxn | 2010_10_21 | Kapa | 24.80 | 200.00 | 4.90 | |
S5 | 96 rxn | 2010_10_21 | Kapa | 26.70 | 200.00 | 5.30 | |
S6 | 96 rxn | 2010_10_22 | Kapa | 27.70 | 200.00 | 5.50 | |
S7 | 96 rxn | 2010_10_22 | Kapa | 32.00 | 200.00 | 6.40 | |
1st prep | Total (ug) | ||||||
S6 | 96 rxn | 2010_10_01 | EconoTaq | 24.60 | 102.00 | 2.50 | 8.00 |
S7 | 96 rxn | 2010_09_27 | Kapa | 36.90 | 200.00 | 7.30 | 13.70 |
Probe set | amount used in PAGE SS (ug) | yields (ug) | %yields | % digestion | total expected complete digestion yields | %recovery |
S1 | 13.00 | 6.50 | 50.00 | 92.00 | 11.96 | 54.35 |
S2 | 15.60 | 7.50 | 48.08 | 92.00 | 14.35 | 52.26 |
S3 | 13.80 | 5.80 | 42.03 | 94.00 | 12.97 | 44.71 |
S4 | 10.70 | 4.90 | 45.79 | 93.00 | 9.95 | 49.24 |
S5 | 9.60 | 5.30 | 55.21 | 92.00 | 8.83 | 60.01 |
S6 | 12.00 | 5.50 | 45.83 | >90 | 10.80 | 50.93 |
S7 | 12.40 | 6.40 | 51.61 | 93.00 | 11.53 | 55.50 |