Noi:Probe preparation data/Probes Prep

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Revision as of 00:54, 9 December 2010 by >Noi
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Summary of DMR220k probe set production

Probe set No. of rxn Date of production Enzyme Conc. (ng/ul) Vol. (ul) Yields (ug) for DNA capture
S1 96 rxn 2010_10_24 Kapa 26.20 250.00 6.50 diluted to 2ng/ul vol 100ul
S2 96 rxn 2010_10_24 Kapa 30.10 250.00 7.50 diluted to 2ng/ul vol 100ul
S3 96 rxn 2010_10_24 Kapa 23.20 250.00 5.80
S4 96 rxn 2010_10_21 Kapa 24.80 200.00 4.90
S5 96 rxn 2010_10_21 Kapa 26.70 200.00 5.30
S6 96 rxn 2010_10_22 Kapa 27.70 200.00 5.50
S7 96 rxn 2010_10_22 Kapa 32.00 200.00 6.40
1st prep Total (ug)
S6 96 rxn 2010_10_01 EconoTaq 24.60 102.00 2.50 8.00
S7 96 rxn 2010_09_27 Kapa 36.90 200.00 7.30 13.70


Probe set amount used in PAGE SS (ug) yields (ug) %yields % digestion total expected complete digestion yields %recovery
S1 13.00 6.50 50.00 92.00 11.96 54.35
S2 15.60 7.50 48.08 92.00 14.35 52.26
S3 13.80 5.80 42.03 94.00 12.97 44.71
S4 10.70 4.90 45.79 93.00 9.95 49.24
S5 9.60 5.30 55.21 92.00 8.83 60.01
S6 12.00 5.50 45.83 >90 10.80 50.93
S7 12.40 6.40 51.61 93.00 11.53 55.50