Rui:LabNotes/SingleCell/2011-1-20

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Revision as of 20:58, 20 January 2011 by >RuiLiu
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1. Measure the concentration of the RNA samples

--------------------------------------------------------------
RNA ID 	 [ng/ul]	260/280 	260/230
-------------------------------------------------------------- 
Hues54 	 414.50	        2.06	        2.01
Hues6-ES	 641.13	        2.12	        2.11
Hues6-EB	 561.99	        2.11	        2.11
--------------------------------------------------------------

2. Examine the integrity of the RNA samples

  • 100ml agarose gel + 8ul SYBR safe

3. Dilute RNA samples in PCR 8-tube strip (-80C)

1) 1:10 2) 1:10 3) 1:4/1:6/1:5.6 --> 1ng/ul 4) 1:5 --> 0.2ng/ul 5) 1:10 --> 0.02ng/ul