Rui:Haplotying
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Progress
Sequencing analysis
- Dr. Zhang's link on haplotyping analysis [1]
- HL095: [2]
- HL101: [3]
- HL108: [4]
Notes
- "Methylation haplotyping on few cells" project: May 27th email [5]
- Human gDNA samples: Kun's stack - the box at the left up corner (-20C), Rui's box (4C) with GC DNA and Africa's DNA.
- Prepare ABD mixture: 9 mL 40% acrylamide, 1 ml Acrylamide/Bis (19:1), 200 mg DATD in 15 mL tube. Shake to dissolve DATD. Use a 3 cc syringe and .22 um filter to 1 mL of ABD into 1.5 mL tube. The rest can be saved at 4C for 1 week
Protocol
2011
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