Calendar/2011-3-28
Jump to navigation
Jump to search
Library Construction on sample 1-21 from Dr. Heller
Qubit Quantification
- dilute 25ul 200X dye with 4975ul buffer
Sample | Concentration in the Qubit | µL used | Dilution | Sample Concentration | ||
1 | 37.2 | ng/mL | 1 | 200 | 7.44 | µg/mL |
2 | 103 | ng/mL | 1 | 200 | 20.6 | µg/mL |
3 | Out Of Range | |||||
4 | 27 | ng/mL | 1 | 200 | 5.4 | µg/mL |
5 | 47.8 | ng/mL | 1 | 200 | 9.56 | µg/mL |
6 | 12.7 | ng/mL | 1 | 200 | 2.55 | µg/mL |
7 | Out Of Range | |||||
8 | Out Of Range | |||||
9 | Out Of Range | |||||
10 | Out Of Range | |||||
11 | Out Of Range | |||||
12 | Out Of Range | |||||
13 | Out Of Range | |||||
14 | Out Of Range | |||||
15 | Out Of Range | |||||
16 | Out Of Range | |||||
17 | Out Of Range | |||||
18 | Out Of Range | |||||
19 | Out Of Range | |||||
20 | Out Of Range | |||||
21 | Out Of Range |
Tagmentation Reaction
- Prior to assembling the reaction, briefly centrifuge the 5X Nextera Reaction Buffer and Nextera Enzyme Mix tubes to assure that the reagents are at the bottom of the tubes.
- Assemble the following reaction components on ice, in the order listed:
x µl Nuclease-Free Water 50 ng Target DNA (in T10E1 Buffer [10 mM Tris-HCl (pH 7.5), 1 mM EDTA]) 4 µl 5X Nextera Reaction Buffer LMW or HMW (see Important Considerations, no. 1, p. 3) 1 µl Nextera Enzyme Mix (Illumina-compatible) 20 µl Total reaction volume