Rui:LabNotes/SingleCell/2011-5-23

From ZhangLabWiki
Jump to navigation Jump to search

Library construct by Nextera

Library construct by ligation with Y adaptor

>Digestion ng/ul 260/280 260/230
sperm RNA 39.12 1.9 2.77
ES RNA 51.57 1.92 1.92
sperm lysis 16.27 1.73 1.42
ES lysis 45.64 1.93 1.99
>Ligation ng/ul 260/280 260/230
sperm RNA 35.03 2.05 2.19
ES RNA 45.66 1.92 1.99
sperm lysis 13.99 2.04 1.7
ES lysis 39.23 2.06 2.12
>PCR ng/ul 260/280 260/230
sperm RNA ID5 21.29 1.73 1.69
ES RNA ID6 21.31 1.72 1.7
sperm lysis ID7 19.24 1.61 1.14
ES lysis ID8 20.03 1.74 1.1

Cloning of PCR product