Brandon Sos
Current Projects
Protocols
AMPure XP beads purification only (for >= 5 ng)
AMPure XP beads purification 2-step PCR (for <=5 ng)
Invitrogen MAXIscript T7 In vitro transcription kit
SMART MMLV RT protocol (for first strand synthesis)
standard PCR cycling for for different enzymes etc
enzyme reaction buffer compositions
tn5 production protocol updated R3 (7/30/2014)
tn5 production protocol updated R4 (9/3/2014)
Nextera_XT_V2_sequences_and_outline
THS-seq_RNA_processing_with_double_stranded_cDNA_and_tn5_fragmentation_adaptor_addition
THS-seq protocol updated to using Nextera XT V2 sequences
- notes
- minielute colums for <10 ul samples, otherwise use qiaquick columns
- N2 adaptor is illuminia adaptor. Nextera adaptor is nextera adaptor
Main Calendar
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<calendar> name=Brandon:LabNotes/Project1 format=%name/%year-%month-%day date=2011/02/01 view=twoyear </calendar>