Rui:LabNotes/Haplotyping/2011-11-4

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Revision as of 23:23, 4 November 2011 by >Sam Chiang (→‎Results)
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Test condition for haplotyping

  • Continue with lab note 11.3.11
  • Tagmentation and PCR

Samples

  • Non-MDA ~1pg cell lysates: from same patch of cells as MDA samples, Ptnase treatment, Nextera
  • MDA samples: 1ul each of all 8 MDA product [17ct-2, 22ct-3, 27ct-3,4, 30ct-1,2,3,4] ranging from 1ng - 20ng

Procedure

  • Tagmentation: 1ul 1:50d Trp-I, 1ul LMW buffer, fill up to 5ul
  • PCR: 15ul Kapa QPCR MM, 0.3ul each B/O primer, fill up to 30ul

Results

MDA samples File:11.4.11 MDA-nextera.jpg non-MDA samples File:11.4.11 ctrls.jpg

gel check: MDA samples are over-amplified except 2 from 27ctFile:11.4.11 gel.jpg